Oxidative stress is a leading driver of ovarian aging. Silent mating-type information regulation 2 homolog-1 (Sirt1) plays an role in ovarian function. Resveratrol has numerous effects, including anti-oxidant and Sirt1 activator. The aim of the study was to investigate the effect of resveratrol on aging-induced ovarian change in rats. The female Sprague Dawley rats were randomly divided into three groups: young control (Con), Aged+Res (20 mg/kg/day resveratrol for 45 days), and Aged. Anti-Mullerian hormone (AMH) was detected by ELISA assay. Malondialdehyde (MDA), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) were detected by conventional method. The ovarian structure and follicles were observed by hematoxylin staining, the caspase-3 and Sirt1 were detected by immunohistochemistry and Western blotting. The AMH in the Aged+Res group was elevated, compared to that in Aged group (p < 0.05). The MDA was decreased and GSH-Px and SOD were increased in the Aged+Res group (p < 0.05). The primordial and primary follicles were increased in the Aged+Res group (p < 0.05). The Sirt1 was increased and caspase-3 was decreased in the Aged+Res group (p < 0.05). These results indicate that resveratrol can delay ovarian aging, probably by reducing oxidative damage and increasing Sirt1.
基金：
Hebei Province Science and Technology Department Fund for Livelihood Science and Technology Special [192777106D]; Hebei Province Key science and technology research plan [ZD20140140]
第一作者机构：[1]Hebei Chest Hosp, Dept Lab Med, Shijiazhuang, Hebei, Peoples R China
通讯作者：
通讯机构：[2]Hebei Med Univ, Hosp 4, Dept Reprod Med, Shijiazhuang, Hebei, Peoples R China[*1]Department of Reproductive Medicine, Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, China
推荐引用方式(GB/T 7714)：
Wu Haifeng,Xue Jianchang,Di Hongqin,et al.Resveratrol improves ovarian function in aged rat by inhibiting oxidative stress and activating the Sirt1[J].GENERAL PHYSIOLOGY AND BIOPHYSICS.2022,41(1):53-61.doi:10.4149/gpb_2021040.
APA：
Wu, Haifeng,Xue, Jianchang,Di, Hongqin,Lv, Cuiting,Hao, Yali&Nie, Zhaoyan.(2022).Resveratrol improves ovarian function in aged rat by inhibiting oxidative stress and activating the Sirt1.GENERAL PHYSIOLOGY AND BIOPHYSICS,41,(1)
MLA：
Wu, Haifeng,et al."Resveratrol improves ovarian function in aged rat by inhibiting oxidative stress and activating the Sirt1".GENERAL PHYSIOLOGY AND BIOPHYSICS 41..1(2022):53-61
