Tandem mass tagging combined with liquid chromatography-tandem mass spectrometry technique to detect protein markers in gastroesophageal junction adenocarcinoma
Background Gastroesophageal junction adenocarcinoma (GEJA) is a malignant tumor located at the junction of the esophagus and stomach, the incidence of which is increasing year by year, while screening for early biomarkers is limited. Tandem mass tagging (TMT) coupled with liquid chromatography-tandem mass spectrometry (LC/MS/MS) has been used to screen for differential proteins in various cancers. Methods Differential proteins in GEJA and precancerous lesions were screened using TMT-LC/MS/MS, and then proteins that met expectations were selected for trend clustering analysis, combined with GO and KEGG analysis for functional annotation of differential proteins in GEJA. Then, parallel reaction monitoring and immunohistochemistry techniques were used to validate the accuracy of the proteomics data. Results Our group screened the differential proteins during GEJA progression using proteomics technology, analyzed the expression trends and functional regions involved in the differential proteins during carcinogenesis, and validated the accuracy of the experimental results. Conclusions The screening of differential proteins in GEJA carcinogenesis based on TMT-LC/MS/MS technology provides detailed information for the elucidation of GEJA progression process, pathogenesis, early screening and screening of candidate markers.
基金:
Science and Technology Foundation
of Hebei Province (2018674).
语种:
外文
被引次数:
WOS:
PubmedID:
中科院分区:
出版当年[2022]版:
大类|3 区化学
小类|3 区光谱学3 区分析化学4 区生化研究方法
最新[2025]版:
大类|4 区化学
小类|4 区生化研究方法4 区分析化学4 区光谱学
JCR分区:
出版当年[2022]版:
Q3CHEMISTRY, ANALYTICALQ3SPECTROSCOPYQ4BIOCHEMICAL RESEARCH METHODS
最新[2024]版:
Q3CHEMISTRY, ANALYTICALQ3SPECTROSCOPYQ4BIOCHEMICAL RESEARCH METHODS
第一作者机构:[1]Hebei Med Univ, Hosp 4, Dept Endoscopy, Shijiazhuang, Hebei, Peoples R China
共同第一作者:
通讯作者:
通讯机构:[4]Hebei Med Univ, Hosp 4, Dept Gastroenterol, 12 Jiankang Rd, Shijiazhuang 050000, Hebei, Peoples R China[*1]Department of Gastroenterology, Fourth Hospital of Hebei Medical University, 12 Jiankang Road, Chang 'an District, Shijiazhuang, Hebei, 050000, China.
推荐引用方式(GB/T 7714):
Zheng Xiuli,Bi Yanna,Yang Tianshuo,et al.Tandem mass tagging combined with liquid chromatography-tandem mass spectrometry technique to detect protein markers in gastroesophageal junction adenocarcinoma[J].RAPID COMMUNICATIONS IN MASS SPECTROMETRY.2022,36(18):e9355.doi:10.1002/rcm.9355.
APA:
Zheng, Xiuli,Bi, Yanna,Yang, Tianshuo,Zhao, Lianmei,Wu, Mingli...&Li, Shengmian.(2022).Tandem mass tagging combined with liquid chromatography-tandem mass spectrometry technique to detect protein markers in gastroesophageal junction adenocarcinoma.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,36,(18)
MLA:
Zheng, Xiuli,et al."Tandem mass tagging combined with liquid chromatography-tandem mass spectrometry technique to detect protein markers in gastroesophageal junction adenocarcinoma".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 36..18(2022):e9355