Proline metabolism reprogramming of trained macrophages induced by early respiratory infection combined with allergen sensitization contributes to development of allergic asthma in childhood of mice
机构：[1]Department of Immunology, Hebei Medical University, Shijiazhuang, China,[2]Key Laboratory of Immune Mechanism and Intervention on Serious Disease in Hebei Province, Hebei Medical University, Shijiazhuang, China,[3]The Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang, China,河北医科大学第四医院[4]Clinical Lab, Hebei Provincial People’s Hospital, Shijiazhuang, China
BackgroundInfants with respiratory syncytial virus (RSV)-associated bronchiolitis are at increased risk of childhood asthma. Recent studies demonstrated that certain infections induce innate immune memory (also termed trained immunity), especially in macrophages, to respond more strongly to future stimuli with broad specificity, involving in human inflammatory diseases. Metabolic reprogramming increases the capacity of the innate immune cells to respond to a secondary stimulation, is a crucial step for the induction of trained immunity. We hypothesize that specific metabolic reprogramming of lung trained macrophages induced by neonatal respiratory infection is crucial for childhood allergic asthma. ObjectiveTo address the role of metabolic reprogramming in lung trained macrophages induced by respiratory virus infection in allergic asthma. MethodsNeonatal mice were infected and sensitized by the natural rodent pathogen Pneumonia virus of mice (PVM), a mouse equivalent strain of human RSV, combined with ovalbumin (OVA). Lung CD11b(+) macrophages in the memory phase were re-stimulated to investigate trained immunity and metabonomics. Adoptive transfer, metabolic inhibitor and restore experiments were used to explore the role of specific metabolic reprogramming in childhood allergic asthma. ResultsPVM infection combined with OVA sensitization in neonatal mice resulted in non-Th2 (Th1/Th17) type allergic asthma following OVA challenge in childhood of mice. Lung CD11b(+) macrophages in the memory phage increased, and showed enhanced inflammatory responses following re-stimulation, suggesting trained macrophages. Adoptive transfer of the trained macrophages mediated the allergic asthma in childhood. The trained macrophages showed metabolic reprogramming after re-stimulation. Notably, proline biosynthesis remarkably increased. Inhibition of proline biosynthesis suppressed the development of the trained macrophages as well as the Th1/Th17 type allergic asthma, while supplement of proline recovered the trained macrophages as well as the allergic asthma. ConclusionProline metabolism reprogramming of trained macrophages induced by early respiratory infection combined with allergen sensitization contributes to development of allergic asthma in childhood. Proline metabolism could be a well target for prevention of allergic asthma in childhood.
基金：
National Natural Science Foundation of China; Natural Science Foundation of Hebei province; [81671635]; [H2020206510]; [H2016206473]
第一作者机构：[1]Department of Immunology, Hebei Medical University, Shijiazhuang, China,
共同第一作者：
通讯作者：
通讯机构：[1]Department of Immunology, Hebei Medical University, Shijiazhuang, China,[2]Key Laboratory of Immune Mechanism and Intervention on Serious Disease in Hebei Province, Hebei Medical University, Shijiazhuang, China,
推荐引用方式(GB/T 7714)：
Li Hanglin,Ma Linyan,Li Wenjian,et al.Proline metabolism reprogramming of trained macrophages induced by early respiratory infection combined with allergen sensitization contributes to development of allergic asthma in childhood of mice[J].FRONTIERS IN IMMUNOLOGY.2022,13:doi:10.3389/fimmu.2022.977235.
APA：
Li, Hanglin,Ma, Linyan,Li, Wenjian,Zheng, Boyang,Wang, Junhai...&Zeng, Ruihong.(2022).Proline metabolism reprogramming of trained macrophages induced by early respiratory infection combined with allergen sensitization contributes to development of allergic asthma in childhood of mice.FRONTIERS IN IMMUNOLOGY,13,
MLA：
Li, Hanglin,et al."Proline metabolism reprogramming of trained macrophages induced by early respiratory infection combined with allergen sensitization contributes to development of allergic asthma in childhood of mice".FRONTIERS IN IMMUNOLOGY 13.(2022)
医学