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Preparation and characterization of nanobodies targeting SARS-CoV-2 papain-like protease

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机构: [1]Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China [2]The CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China [3]University of Chinese Academy of Sciences, Beijing, 100049, China [4]State Key Laboratory of Drug Research & Center of Pharmaceutics, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China [5]Department of Pharmacy, National Cancer Center, National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China [6]National Cancer Center, National Clinical Research Center for Cancer/Hebei Cancer Hospital, Chinese Academy of Medical Sciences, Langfang, 065001, China [7]Shandong Provincial Key Laboratory of Biopharmaceuticals, Shandong Academy of Pharmaceutical Sciences, Jinan, 250101, China
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关键词: SARS-CoV-2 Papain like protease (PLpro) Nanobody PLpro inhibitor

摘要:
Coronavirus Papain-like protease (PLpro) mediates the cleavage of viral polyproteins and assists the virus escaping from innate immune response. Thus, PLpro is an attractive target for the development of broad-spectrum drugs as it has a conserved structure across different coronaviruses. In this study, we purified SARS-CoV-2 PLpro as an immune antigen, constructed a nanobody phage display library, and identified a set of nanobodies with high affinity for SARS-CoV-2. In addition, enzyme activity experiments demonstrated that two nanobodies had a significant inhibitory effect on the PLpro. These nanobodies should therefore be investigated as candidates for the treatment of coronaviruses.Copyright © 2023 Elsevier Inc. All rights reserved.

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出版当年[2023]版:
大类 | 4 区 生物学
小类 | 4 区 生化研究方法 4 区 生化与分子生物学 4 区 生物工程与应用微生物
最新[2025]版:
大类 | 4 区 生物学
小类 | 4 区 生化研究方法 4 区 生化与分子生物学 4 区 生物工程与应用微生物
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出版当年[2023]版:
Q4 BIOCHEMICAL RESEARCH METHODS Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
最新[2023]版:
Q4 BIOCHEMICAL RESEARCH METHODS Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

影响因子: 最新[2023版] 最新五年平均 出版当年[2023版] 出版当年五年平均 出版前一年[2022版]

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第一作者机构: [1]Department of Biopharmaceutics, College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China
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通讯作者:
通讯机构: [2]The CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China [3]University of Chinese Academy of Sciences, Beijing, 100049, China [5]Department of Pharmacy, National Cancer Center, National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China [6]National Cancer Center, National Clinical Research Center for Cancer/Hebei Cancer Hospital, Chinese Academy of Medical Sciences, Langfang, 065001, China [*1]The CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China. [*2]Department of Pharmacy, National Cancer Center/National Clinical Research Center for Cancer, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China.
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