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EZH2 regulates pancreatic cancer cells through E2F1, GLI1, CDK3, and Mcm4

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WOS体系：
Pubmed体系：

收录情况： ◇ SCIE

作者：
Li Hongfeng[1] * ; Wang Hailong[2] * ; Cui Yunlong[3] * ; Jiang Wenhua[4] ; Zhan Hongjie[5] ; Feng Lixia[6] ; Gao Mingyou[7] ; Zhao Kuo[7] ; Zhang Limeng[7] ; Xie Xiaojing[7] ; Zhao Ning[7] ; Li Ying[8] ; Liu Pengfei[2] ;
机构： [1]Department of Clinical Laboratory, Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, Tianjin 300120, China [2]Department of Oncology, Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, No. 354 Beima Road, Hongqiao District, Tianjin 300120, China [3]Department of Hepatobiliary Oncology, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy of Tianjin, Tianjin’s Clinical Research Center for Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China [4]Department of Radiotherapy, The Second Hospital of Tianjin Medical University, Tianjin 300211, China [5]Department of Gastric Cancer, National Clinical Research Center of Cancer, Key Laboratory of Cancer Prevention and Therapy of Tianjin, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China [6]Department of Nursing, National Clinical Research Center of Cancer, Key Laboratory of Cancer Prevention and Therapy of Tianjin, Tianjin Medical University Cancer Institute and Hospital, Konggang Hospital, Tianjin 300300, China [7]Department of Oncology, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy of Tianjin, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China [8]Department of Medical Oncology, The Fourth Hospital of Hebei Medical University, No. 12 Health Road, Shijiazhuang 050000, Hebei, China
出处：
DOI： 10.1186/s41065-023-00280-1
ISSN：

关键词： PC EZH2 Proliferation Migration Transcriptome

摘要：
Pancreatic cancer (PC) is one of the most common malignant tumors in digestive tract. To explore the role of epigenetic factor EZH2 in the malignant proliferation of PC, so as to provide effective medical help in PC. Sixty paraffin sections of PC were collected and the expression of EZH2 in PC tissues was detected by immunohistochemical assay. Three normal pancreas tissue samples were used as controls. The regulation of EZH2 gene on proliferation and migration of normal pancreatic cell and PC cell were determined by MTS, colony forming, Ki-67 antibody, scratch and Transwell assays. Through differential gene annotation and differential gene signaling pathway analysis, differentially expressed genes related to cell proliferation were selected and verified by RT-qPCR. EZH2 is mainly expressed in the nuclei of pancreatic tumor cells, but not in normal pancreatic cells. The results of cell function experiments showed that EZH2 overexpression could enhance the proliferation and migration ability of PC cell BXPC-3. Cell proliferation ability increased by 38% compared to the control group. EZH2 knockdown resulted in reduced proliferation and migration ability of cells. Compared with control, proliferation ability of cells reduced by 16%-40%. The results of bioinformatics analysis of transcriptome data and RT-qPCR demonstrated that EZH2 could regulate the expression of E2F1, GLI1, CDK3 and Mcm4 in normal and PC cells. The results revealed that EZH2 might regulate the proliferation of normal pancreatic cell and PC cell through E2F1, GLI1, CDK3 and Mcm4.© 2023. The Author(s).

基金：
This work was supported by the National Natural Science Foundation of China (grant numbers 81702098) and Clinical Medical Research Project of Tianjin Medical University (grant numbers 2017kylc004).
语种：
外文
被引次数：
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PubmedID：
中科院分区：
出版当年[2023]版：
大类 | 3 区 生物学
小类 | 4 区 遗传学
最新[2025]版：
大类 | 4 区 生物学
小类 | 4 区 遗传学
JCR分区：
出版当年[2023]版：
Q3 GENETICS & HEREDITY
最新[2023]版：
Q3 GENETICS & HEREDITY

影响因子： 最新[2023版] 最新五年平均 出版当年[2023版] 出版当年五年平均 出版前一年[2022版]

第一作者：
第一作者机构： [1]Department of Clinical Laboratory, Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, Tianjin 300120, China
共同第一作者：
通讯作者：
通讯机构： [8]Department of Medical Oncology, The Fourth Hospital of Hebei Medical University, No. 12 Health Road, Shijiazhuang 050000, Hebei, China
推荐引用方式(GB/T 7714)：
Li Hongfeng,Wang Hailong,Cui Yunlong,et al.EZH2 regulates pancreatic cancer cells through E2F1, GLI1, CDK3, and Mcm4[J].HEREDITAS.2023,160(1):doi:10.1186/s41065-023-00280-1.
APA：
Li Hongfeng,Wang Hailong,Cui Yunlong,Jiang Wenhua,Zhan Hongjie...&Liu Pengfei.(2023).EZH2 regulates pancreatic cancer cells through E2F1, GLI1, CDK3, and Mcm4.HEREDITAS,160,(1)
MLA：
Li Hongfeng,et al."EZH2 regulates pancreatic cancer cells through E2F1, GLI1, CDK3, and Mcm4".HEREDITAS 160..1(2023)

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