This study aims to examine the impacts of Scutellaria strigillosa Hemsl. (SSH) on the proliferation, apoptosis of human hepatoma cell HepG2 and screen the bioactive components. We found that SSH extract inhibited HepG2 proliferation, arrested cell division prior to S phase. Additionally, SSH extract exposure induced apoptosis, and increased the proportions of late apoptotic cells. Specifically, we focus on the inhibitory effect of SSH extract on aspartate (3-hydroxylase, a key therapeutic target of hepatocellular carcinoma closely related with the proliferation and apoptosis of HepG2. We found SSH extract with notable inhibitory activity against aspartate (3-hydroxylase, elucidated the main bioactive constituents by HPLC-Q-TOF/MS and Molecular docking analysis. In conclusion, these results provided the antiproliferative and proapoptotic effects of SSH on HepG2 cell, elucidated the main bioactive constituents based on aspartate (3-hydroxylase inhibition. These data revealed the potential value of SSH and its bioactive components for the prevention and treatment of liver cancer for the first time.& COPY; 2023 Elsevier Inc. All rights reserved.
基金：
National Natural Science Foundation of China [31800975]; Science and Technology Project of Hebei Education Department [ZD2020141]; Hebei Natural Science Foundation [H2022206517]; Project of Hebei Traditional Chinese Medicine Administration [2023131]; Project of Department of Health of Hebei Province [20221259, 20231548]
第一作者机构：[1]Hebei Med Univ, Zhongshan Rd 361, Shijiazhuang 050017, Peoples R China
共同第一作者：
通讯作者：
通讯机构：[2]Hebei Med Univ, Affiliated Hosp 4, Hlth Rd 12, Shijiazhuang 050011, Peoples R China
推荐引用方式(GB/T 7714)：
Wang Hairong,Liu Yuan,Cui Jiawen,et al.Effects of Scutellaria strigillosa Hemsl. extract on HepG2 cell proliferation and apoptosis through binding to aspartate (3-hydroxylase[J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2023,668:62-69.doi:10.1016/j.bbrc.2023.05.077.
APA：
Wang, Hairong,Liu, Yuan,Cui, Jiawen,Tong, Miaomiao,Guan, Wenlong...&Zhao, Lili.(2023).Effects of Scutellaria strigillosa Hemsl. extract on HepG2 cell proliferation and apoptosis through binding to aspartate (3-hydroxylase.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,668,
MLA：
Wang, Hairong,et al."Effects of Scutellaria strigillosa Hemsl. extract on HepG2 cell proliferation and apoptosis through binding to aspartate (3-hydroxylase".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 668.(2023):62-69
