详情页

当前位置：首页 > 详情页

M2 Macrophage-Derived Extracellular Vesicles Containing microRNA-501-3p Promote Colon Cancer Progression through the SETD7/DNMT1/SOCS3 Axis

43| 导出 | 链接全文 |

文献详情

资源类型：

WOS体系：

Pubmed体系：

收录情况： ◇ SCIE

作者：

机构： [1]The Second Department of General Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, P. R. China. [2]Department of Gastroenterology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, P. R. China. [3]The Second Department of General Surgery, Fengning Manchu Autonomous County Hospital, Chengde, P. R. China.

出处：

DOI：

ISSN：

摘要：

Macrophage-derived extracellular vesicles with microRNAs can cause and develop colon cancer.We investigated M2 macrophage-derived extracellular vesicles and colon cancer.A prospective and experimental study of M2 macrophage-derived extracellular vesicles in colon cancer.This study was completed at The Fourth Hospital of Hebei Medical University.Colon cancer patients who had undergone surgical resection.Suppressor of cytokine signaling 3, miR-501-3p, SET domain-containing 7, and DNA methyltransferase 1 were measured in colon cancer samples. Multiple experiments determined suppressor of cytokine signaling 3, miR-501-3p, SET domain containing 7, and DNA methyltransferase 1 binding affinity. M2 macrophages were cultivated from M0 macrophages isolated from healthy donor PBMCs and polarized to produce extracellular vesicles. Gain- or loss-of-function tests using colon cancer cells and M2 macrophage-derived extracellular vesicles revealed cell biological processes. Finally, animal models were created to test how miR-501-3p from M2-extracellular vesicles affects tumor growth via the SET domain containing 7/DNA methyltransferase 1/suppressor of cytokine signaling 3.Colon cancer increased miR-501-3p and DNA methyltransferase 1 and downregulated suppressor of cytokine signaling 3 and SET domain containing 7. miR-151-3p inhibited SET domain-containing 7, upregulating DNA methyltransferase 1. Increased promoter methylation by DNA methyltransferase 1 decreased suppressor of cytokine signaling 3 expression. M2-EVs with miR-501-3p regulated the SET domain containing 7/DNA methyltransferase 1/suppressor of cytokine signaling 3 axis to induce apoptosis and colon cancer cell growth, invasion, and migration. M2-EV-delivered miR-501-3p also regulated the SET domain containing 7/DNA methyltransferase 1/suppressor of cytokine signaling 3 axis to promote tumor growth in animals.Further research is needed in clinical application of M2 macrophage-derived extracellular vesicles containing miR-501-3p as a biomarker of colon cancer.M2 macrophage-derived extracellular vesicles with miR-501-3p regulate the SET domain containing 7/DNA methyltransferase 1/suppressor of cytokine signaling 3 axis to promote colon cancer.Copyright © The ASCRS 2023.

基金：

语种：

被引次数：

WOS：

PubmedID：

中科院分区：

出版当年[2023]版：

大类 | 2 区医学

小类 | 2 区外科 3 区胃肠肝病学

最新[2025]版：

大类 | 2 区医学

小类 | 2 区外科 3 区胃肠肝病学

JCR分区：

出版当年[2023]版：

Q1 SURGERY Q2 GASTROENTEROLOGY & HEPATOLOGY

最新[2024]版：

Q1 SURGERY Q2 GASTROENTEROLOGY & HEPATOLOGY

影响因子： 3.7 最新[2024版] 3.8 最新五年平均 3.3 出版当年[2023版] 4 出版当年五年平均 4.1 出版前一年[2022版] 3.7 出版后一年[2024版]

第一作者：

第一作者机构： [1]The Second Department of General Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, P. R. China.

通讯作者：

通讯机构： [1]The Second Department of General Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, P. R. China. [*1]The Second Department of General Surgery, The Fourth Hospital of Hebei Medical University, No.12, Jiankang Road, Shijiazhuang 050011, Hebei Province, P. R. China

推荐引用方式(GB/T 7714)：

APA：

MLA：