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TRAF6 Induces the Activation of the PI3K/NF-κB Signaling Pathway in Non-Hodgkin's Lymphoma

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机构: [1]Hebei Med Univ, Hosp 4, Dept Pathol, Shijiazhuang 050035, Hebei, Peoples R China [2]Shijiazhuang Peoples Hosp, Resp Dept, Shijiazhuang 050000, Hebei, Peoples R China [3]Hebei Gen Hosp, Dept Pediat, Shijiazhuang 050051, Hebei, Peoples R China [4]Hebei Med Univ, Dept Pathol, Hosp 4, Shijiazhuang 050035, Hebei, Peoples R China
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关键词: TRAF6 non-Hodgkin's lymphoma (NHL) PI3K NF-KB

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Background: Lymphoma has emerged as the most common neoplasm among children, nearly half of them being non-Hodgkin lymphoma (NHL). To explore appropriate therapeutic targets and suggest directions for future research, this study aimed to identify the effects of TNF receptor-associated factor 6 (TRAF6) on NHL and its correlation with the phosphatidylinositol 3-kinase and nuclear factor kappa-B (PI3K/NF-KB) pathway.Methods: Blood samples were collected from healthy children and those affected with NHL. The cell lines, Raji human B lymphoma (iCell-h178) and U-937 human leukemia (iCell-h267), were maintained on RPMI-1640 culture medium. The expression profile of TRAF6, phosphatidylinositol 3-kinase (PI3K), and nuclear factor kappa-B (NF -KB) was determined by performing the real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) using blood samples from both healthy and affected children. Furthermore, RT-qPCR, Western Blot, Cell Counting Kit-8 (CCK-8) assay, and flow cytometry were used to evaluate the impact of TRAF6 expression levels on NHL cell proliferation and apoptosis, as well as the role of TRAF6 in mediating the PI3K/NF-KB pathway. Results: TRAF6, PI3K, and NF -KB were over-expressed in NHL patients (p < 0.05). Decreased/increased TRAF6 expression led to decreased/increased Proliferating cell nuclear antigen (PCNA), Cyclin D1, Bcl-2 protein expression and NHL cell proliferation (p < 0.05), while increasing/decreasing Cleaved Caspase-3, Bax protein expressions and NHL cell apoptosis (p < 0.05). In addition, decreased/increased TRAF6 expression could lead to decreased/increased p-PI3K, p-NF -KB protein expressions (p < 0.05). Conclusion: In summary, TRAF6 was over-expressed in NHL patients and NHL cell lines. However, down-regulation of TRAF6 reduced proliferation and induced apoptosis in NHL cells through deactivating PI3K/NF-KB pathway, thus suppressing the progression of NHL.

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大类 | 4 区 医学
小类 | 4 区 内分泌学与代谢 4 区 免疫学 4 区 医学:研究与实验 4 区 生理学
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Q4 ENDOCRINOLOGY & METABOLISM Q4 IMMUNOLOGY Q4 MEDICINE, RESEARCH & EXPERIMENTAL Q4 PHYSIOLOGY
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第一作者机构: [1]Hebei Med Univ, Hosp 4, Dept Pathol, Shijiazhuang 050035, Hebei, Peoples R China
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通讯机构: [1]Hebei Med Univ, Hosp 4, Dept Pathol, Shijiazhuang 050035, Hebei, Peoples R China [4]Hebei Med Univ, Dept Pathol, Hosp 4, Shijiazhuang 050035, Hebei, Peoples R China
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