Background: Lymphoma has emerged as the most common neoplasm among children, nearly half of them being non-Hodgkin lymphoma (NHL). To explore appropriate therapeutic targets and suggest directions for future research, this study aimed to identify the effects of TNF receptor-associated factor 6 (TRAF6) on NHL and its correlation with the phosphatidylinositol 3-kinase and nuclear factor kappa-B (PI3K/NF-KB) pathway.Methods: Blood samples were collected from healthy children and those affected with NHL. The cell lines, Raji human B lymphoma (iCell-h178) and U-937 human leukemia (iCell-h267), were maintained on RPMI-1640 culture medium. The expression profile of TRAF6, phosphatidylinositol 3-kinase (PI3K), and nuclear factor kappa-B (NF -KB) was determined by performing the real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) using blood samples from both healthy and affected children. Furthermore, RT-qPCR, Western Blot, Cell Counting Kit-8 (CCK-8) assay, and flow cytometry were used to evaluate the impact of TRAF6 expression levels on NHL cell proliferation and apoptosis, as well as the role of TRAF6 in mediating the PI3K/NF-KB pathway. Results: TRAF6, PI3K, and NF -KB were over-expressed in NHL patients (p < 0.05). Decreased/increased TRAF6 expression led to decreased/increased Proliferating cell nuclear antigen (PCNA), Cyclin D1, Bcl-2 protein expression and NHL cell proliferation (p < 0.05), while increasing/decreasing Cleaved Caspase-3, Bax protein expressions and NHL cell apoptosis (p < 0.05). In addition, decreased/increased TRAF6 expression could lead to decreased/increased p-PI3K, p-NF -KB protein expressions (p < 0.05). Conclusion: In summary, TRAF6 was over-expressed in NHL patients and NHL cell lines. However, down-regulation of TRAF6 reduced proliferation and induced apoptosis in NHL cells through deactivating PI3K/NF-KB pathway, thus suppressing the progression of NHL.
基金:
Key Science and Tech-nology Research Plan [20230915]
语种:
外文
WOS:
中科院分区:
出版当年[2023]版:
大类|4 区医学
小类|4 区内分泌学与代谢4 区免疫学4 区医学:研究与实验4 区生理学
最新[2025]版:
无
JCR分区:
出版当年[2023]版:
Q4ENDOCRINOLOGY & METABOLISMQ4IMMUNOLOGYQ4MEDICINE, RESEARCH & EXPERIMENTALQ4PHYSIOLOGY
第一作者机构:[1]Hebei Med Univ, Hosp 4, Dept Pathol, Shijiazhuang 050035, Hebei, Peoples R China
通讯作者:
通讯机构:[1]Hebei Med Univ, Hosp 4, Dept Pathol, Shijiazhuang 050035, Hebei, Peoples R China[4]Hebei Med Univ, Dept Pathol, Hosp 4, Shijiazhuang 050035, Hebei, Peoples R China
推荐引用方式(GB/T 7714):
Li Yang,Wang Chuan,Wang Jiangya,et al.TRAF6 Induces the Activation of the PI3K/NF-κB Signaling Pathway in Non-Hodgkin's Lymphoma[J].JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS.2023,37(11):5903-5911.doi:10.23812/j.biol.regul.homeost.agents.20233711.565.
APA:
Li, Yang,Wang, Chuan,Wang, Jiangya&Yang, Xiangdan.(2023).TRAF6 Induces the Activation of the PI3K/NF-κB Signaling Pathway in Non-Hodgkin's Lymphoma.JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS,37,(11)
MLA:
Li, Yang,et al."TRAF6 Induces the Activation of the PI3K/NF-κB Signaling Pathway in Non-Hodgkin's Lymphoma".JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS 37..11(2023):5903-5911