机构：[1]Department of Clinical Nutrition, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei 050051, China.[2]School of Public Health, Hebei Medical University, Shijiazhuang, Hebei 050017, China.[3]Department of Nutrition, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, China.医技科室临床营养科河北医科大学第四医院[4]Department of Research, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei 050051, China.
The purpose of this study is to observe LP45 (Lactobacillus plantarum 45) to investigate the mechanism by which LP45 attenuates oxidative stress-induced damage and regulates the osteoblast-osteoclast balance.The oxidative stress level and osteoblast- and osteoclast-related proteins were detected by immunofluorescence staining, Western blotting, ROS fluorescent probe and ELISA. Osteoblast cell proliferation capacity was determined by the CCK-8 assay. X-ray observation and HE staining were used to detect the effect of LP45 on osteoporosis.The expression level of SHP2 and Src was significantly increased, and the expression levels of NOX4, P22, P47, IL-1β, NLRP3, IRF3, RANK, β-catenin and INF-β were inhibited in LP45 group and LPS + LP45 group as compared to those in LPS group. Compared with that in LPS group, the concentration of SOD was increased and the concentration of MDA was decreased in LPS + LP45 group. The protein expressions of OPG, RANKL, RUNX3, RANK and β-catenin in LP45 group and LPS + LP45 group increased. The protein expressions of NF-κB, CREB and AP-1 in LP45 group and LPS + LP45 group decreased significantly. The results were also confirmed by immunofluorescence staining and ROS fluorescent probe. X-ray observation and HE staining showed that LP45 could inhibit the progression of osteoporosis.LP45 can exert its antioxidant effect by inhibiting the production of oxidative stress to activate the SHP2 signaling pathway, thus promoting osteoblast differentiation and repressing osteoclast formation to maintain bone homeostasis and improve bone metabolism.
基金：
This work was supported by the 2022 Government-Funded Training Program for Clinical Medical Talents.
第一作者机构：[1]Department of Clinical Nutrition, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei 050051, China.
通讯作者：
通讯机构：[1]Department of Clinical Nutrition, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei 050051, China.[2]School of Public Health, Hebei Medical University, Shijiazhuang, Hebei 050017, China.
推荐引用方式(GB/T 7714)：
Yang Yaming,Yan Zheng,Xie Qi,et al.Lactobacillus plantarum 45 activates SHP2 through inhibition of oxidative stress to regulate osteoblast and osteoclast differentiation[J].AGING-US.2024,16(7):6334-6347.doi:10.18632/aging.205708.
APA：
Yang Yaming,Yan Zheng,Xie Qi,Wang Yong,Liu Zhiying&Lei Min.(2024).Lactobacillus plantarum 45 activates SHP2 through inhibition of oxidative stress to regulate osteoblast and osteoclast differentiation.AGING-US,16,(7)
MLA：
Yang Yaming,et al."Lactobacillus plantarum 45 activates SHP2 through inhibition of oxidative stress to regulate osteoblast and osteoclast differentiation".AGING-US 16..7(2024):6334-6347
