This study investigated the regulatory impact of Toll-like receptor 4 (TLR4) gene on glioma cell proliferation and apoptosis, elucidating the molecular mechanisms underlying TLR4-induced growth inhibition in vivo. U-87MG-Sh and U-87MG-NC cells, with silenced TLR4 and negative control plasmid respectively, were established. Eighteen nude mice, divided into transfection, negative control, and blank control groups, were inoculated with corresponding cells. Over four weeks, the transfection group exhibited significantly reduced tumor growth rates, smaller mass and volume, and lower growth activity compared to controls. Histological analysis revealed sparse tumor cells, increased fibrous connective tissue, and slower angiogenesis in the transfection group. Flow cytometry demonstrated a lower proliferation index and increased G0/1 cell count in the transfection group. mRNA levels of TLR4, NF-κB, and CyclinD1 were significantly lower in the transfection group. TLR4 silencing correlated with U-87MG cell proliferation regulation, growth inhibition, NF-κB and CyclinD1 modulation, and induction of cell cycle arrest and apoptosis. These findings suggest TLR4 as a potential gene therapy target for glioma.
基金：
This work was supported by the Hebei Provincial Government funded Clinical Medical Talents Training Project,
ZF2023064 (Study on the mechanism of TLR4 regulating
activation protein-1 pathway to inhibit glioma cell growth
and invasion); Hebei Province Geriatric Disease Prevention Project, 361006 (Study on the regulation of hsa-mir-
335-5p/TLR4 by GLIDR in elderly patients with brain
glioma); Key Science and Technology Research Program
of Hebei Provincial Health Commission, 20221318 (Study
on the mechanism of TLR4 regulating activation protein-1
to inhibit the growth and invasion of glioma cells).
语种：
外文
PubmedID：
第一作者：
第一作者机构：[1]The Fourth Hospital of Hebei Medical University, Shijiazhuang, China
推荐引用方式(GB/T 7714)：
An Zhansen,Zheng Cuihong,Liu Liang,et al.Growth inhibition of subcutaneous tumor with glioma cells in nude mice by silencing TLR4[J].Cellular And Molecular Biology (Noisy-Le-Grand, France).2024,70(6):211-216.doi:10.14715/cmb/2024.70.6.32.
APA：
An Zhansen,Zheng Cuihong,Liu Liang,He Zefeng,Yang Xuan...&Liu Yingzi.(2024).Growth inhibition of subcutaneous tumor with glioma cells in nude mice by silencing TLR4.Cellular And Molecular Biology (Noisy-Le-Grand, France),70,(6)
MLA：
An Zhansen,et al."Growth inhibition of subcutaneous tumor with glioma cells in nude mice by silencing TLR4".Cellular And Molecular Biology (Noisy-Le-Grand, France) 70..6(2024):211-216
