机构：[1]Xingtai People’s Hospital Postdoctoral Workstation, Hebei Province Xingtai People’s Hospital, Xingtai, 054031, China[2]Department of Orthopedics, The Fourth Hospital of Hebei Medical University, Shijiazhuang, 050011, China河北医科大学第四医院[3]Department of Research and Education, Hebei Province Xingtai People’s Hospital, Xingtai, 054031, China[4]Department of Gynecology, Hebei Medical University Second Affiliated Hospital, Shijiazhuang, 050000, China[5]Department of Emergency, Hebei Medical University Second Affiliated Hospital, Shijiazhuang, 050000, China[6]Department of Orthopedics, Xingtai People’s Hospital, Xingtai, 054031, China
Osteosarcoma (OS) is a primary high-grade malignant bone neoplasm, and the prognosis ofOS remains poor due to early metastasis. Leptin plays an essential role in tumorigenesis, but the role of leptin in the development of OS is still not fully understood. In this study, we used a human osteosarcomaMG-63 cell line as an experimentalmodel. MG-63 cells were treated with leptin, and cell proliferation, apoptosis, adhesion, invasion, and gene expression, were evaluated. The results showed that leptin promoted proliferation, decreased adhesion, suppressed apoptosis, and promoted invasion, of MG-63 cells. Moreover, the expression of SIRT1 was upregulated in MG-63 cells exposed to leptin. Furthermore, MMP-2, 8, and 9 were significantly upregulated by SIRT1, while SIRT1 knockdown inhibited the proliferation and invasion of MG-63 cells. In conclusion, our results suggest that leptin promotes OS cell proliferation and invasion by inducing the expression of SIRT1.
基金：
This work was supported by the Key
Project of Medical-Science Research of Hebei Province
[20180564] and the Project of the Natural Science
Foundation of Hebei Province [H2019206309].
第一作者机构：[1]Xingtai People’s Hospital Postdoctoral Workstation, Hebei Province Xingtai People’s Hospital, Xingtai, 054031, China[2]Department of Orthopedics, The Fourth Hospital of Hebei Medical University, Shijiazhuang, 050011, China
通讯作者：
通讯机构：[1]Xingtai People’s Hospital Postdoctoral Workstation, Hebei Province Xingtai People’s Hospital, Xingtai, 054031, China[6]Department of Orthopedics, Xingtai People’s Hospital, Xingtai, 054031, China
推荐引用方式(GB/T 7714)：
Feng Helin,Zhang Xiaochong,Zhang Qianqian,et al.Leptin promotes proliferation and invasion of osteosarcoma cells by upregulating the expression of SIRT1[J].BIOCELL.2020,44(3):443-450.doi:10.32604/biocell.2020.010705.
APA：
Feng, Helin,Zhang, Xiaochong,Zhang, Qianqian,Li, Ze&Zhao, Lili.(2020).Leptin promotes proliferation and invasion of osteosarcoma cells by upregulating the expression of SIRT1.BIOCELL,44,(3)
MLA：
Feng, Helin,et al."Leptin promotes proliferation and invasion of osteosarcoma cells by upregulating the expression of SIRT1".BIOCELL 44..3(2020):443-450
