Laryngeal squamous cell carcinoma (LSCC) is one of the most common malignant tumors in the head and neck area. Melanoma-associated antigens A (MAGE-A) are strictly tumor-specific and are expressed in several types of tumors. To date, no studies have reported the potential ofMAGE-Agenes as markers for circulating tumor cells (CTCs) in patients with LSCC. The present study aimed to evaluate the expression and the possible prognostic significance ofMAGE-Ain the peripheral blood of patients with LSCC. In the present study, the expression ofMAGE-Agenes was determined by multiplex semi-nested PCR and restriction endonuclease treatment of the peripheral blood of patients with LSCC. The association betweenMAGE-Agene expression and clinicopathological parameters and prognosis was evaluated. The results demonstrated that the expression ofMAGE-Awas associated with the predictors that indicate poor prognosis. The expression levels ofMAGE-Aand each individualMAGE-Agene were also associated with a shorter overall survival time of patients with LSCC. In conclusion, the results of the present study suggested that the expression ofMAGE-Agenes may be a potential prognostic marker for patients with LSCC.