The development and validation of an HPLC-MS/MS method for the determination of eriocitrin in rat plasma and its application to a pharmacokinetic study
机构:[1]Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, P.R. China.[2]Department of Pharmacy, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, P.R. China药学部药学部河北医科大学第四医院
Eriocitrin is one of the major active constituents of lemon fruit, and it possesses strong antioxidant, lipid-lowering, anticancer and anti-inflammatory activities and has long been used in food, beverages and wine. In this study, for the first time, a rapid, selective, and sensitive liquid chromatography-tandem mass spectrometry method (LC/MS/MS) with protein precipitation was developed and validated for the analysis of eriocitrin in rat plasma. Chromatographic separation was achieved using a mobile phase, comprising 0.1% formic acid aqueous solution and acetonitrile eluted at a flow rate of 0.8 mL min(-1). In multiple reaction monitoring (MRM) modes, eriocitrin and internal standard (IS) were quantified using precursor-to-product ion transitions of m/z 595.4 -> 287.1 and m/z 252.0 -> 155.9, respectively. The intra- and inter-day precision (RSD) were below 6.79% in plasma, while accuracy (RE) was within +/- 7.67%. The matrix effect, recovery and stability were also demonstrated to be within acceptable limits. This method was successfully employed in the pharmacokinetic study on rats after the oral administration of eriocitrin. The pharmacokinetic parameters show that the maximum plasma concentration (C-max) of eriocitrin was 299.833 +/- 16.743 mu g L-1, while the corresponding time to reach C-max(T-max) was 0.094 +/- 0.019 h, and the half-time (T-1/2) was 1.752 +/- 0.323 h. The present results would be valuable for further research and development of eriocitrin.
基金:
The project was nancially supported by the National Natural
Science Foundation of China (No. 81473180).
第一作者机构:[1]Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, P.R. China.
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推荐引用方式(GB/T 7714):
Li Luya,Feng Rui,Feng Xue,et al.The development and validation of an HPLC-MS/MS method for the determination of eriocitrin in rat plasma and its application to a pharmacokinetic study[J].RSC ADVANCES.2020,10(18):10552-10558.doi:10.1039/c9ra10925k.
APA:
Li, Luya,Feng, Rui,Feng, Xue,Chen, Yuting,Liu, Xin...&Zhang, Lantong.(2020).The development and validation of an HPLC-MS/MS method for the determination of eriocitrin in rat plasma and its application to a pharmacokinetic study.RSC ADVANCES,10,(18)
MLA:
Li, Luya,et al."The development and validation of an HPLC-MS/MS method for the determination of eriocitrin in rat plasma and its application to a pharmacokinetic study".RSC ADVANCES 10..18(2020):10552-10558