Epidermal growth factor receptor (EGFR) is an important gene in the development of lung cancer. Non-small cell lung cancer (NSCLC) is the most common lung cancer. In the present study, the expression of EGFR in 717 patients with NSCLC was detected by Ventana automatic immunohistochemical technique, and the samples was verified by Real-time PCR, and then the results were compared with the data acquired by next-generation sequencing technology (NGS), which is the high throughput, multiple sites for EGFR gene mutation testing. The expression of Ventana EGFR in 717 cases of NSCLC was detected by immunohistochemistry, and the positive rate was 60.70 % (435 / 717). The mutation rate of EGFR was 57.60 % (413/717). The coincidence rate of Ventana EGFR immunohistochemical assay and Real-time PCR assay reached 94.94 %, and the two had high consistency. The coincidence rate of Ventana EGFR immunohistochemical assay and NGS were high correlation. Based on these results, Ventana EGFR automatic immunohistochemical detection has high accuracy, simple operation process, low price and easy interpretation. It can be used as the preferred method for EGFR detection.
基金：
Project supported by Medical Science Research Key Project Fund of
Hebei Province China (No. 20190718).
第一作者机构：[1]Department of Pathology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Pathology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, China[*1]Departments of Pathology, The Fourth Hospital of Hebei Medical University, No.12, Jiankang Road, Shijiazhuang, 050011, China.
推荐引用方式(GB/T 7714)：
Fan Xiaojie,Wang Xiaoxiao,Zhang Meng,et al.Comparison detection methods for EGFR in formalin-fixed paraffin-embedded tissues of patients with NSCLC[J].PATHOLOGY RESEARCH AND PRACTICE.2020,216(1):doi:10.1016/j.prp.2019.152783.
APA：
Fan, Xiaojie,Wang, Xiaoxiao,Zhang, Meng,Deng, Huiyan&Liu, Yueping.(2020).Comparison detection methods for EGFR in formalin-fixed paraffin-embedded tissues of patients with NSCLC.PATHOLOGY RESEARCH AND PRACTICE,216,(1)
MLA：
Fan, Xiaojie,et al."Comparison detection methods for EGFR in formalin-fixed paraffin-embedded tissues of patients with NSCLC".PATHOLOGY RESEARCH AND PRACTICE 216..1(2020)
