机构：[1]Department of General Surgery, Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang, Hebei 050011, China河北医科大学第四医院外二科临床科室[2]Department of General Surgery, Shijiazhuang Traditional Chinese Medical Hospital, Shijiazhuang, Hebei 050051, China[3]Department of Endocrinology, Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang, Hebei 050011, China临床科室内分泌科河北医科大学第四医院
Objective. To investigate whether the polymorphisms of interleukin-12B (IL-12B) were associated with the risk of developing colorectal cancer (CRC). Patients and Methods. Genotypes of rs17860508 and rs3212227 were determined by polymerase chain reaction with a direct sequencing method in 329 CRC patients and 342 matched healthy control subjects. The expression of IL-12B mRNA was determined by RT-qPCR in 50 pairs of CRC tissues and their adjacent normal tissues. Results. Compared with TTAGAG/TTAGAG genotype of rs17860508, the GC/GC and TTAGAG/GC genotypes may significantly increase the risk of CRC (OR = 1.81, 95% CI = 1.18-2.78; OR = 1.46, 95% CI = 1.01-2.12, respectively). Furthermore, the mRNA levels of IL-12B were significantly higher in the CRC tissues from patients with the rs17860508 GC/GC genotype than those with the TTAGAG/GC (P=0.009) and TTAGAG/TTAGAG (P=0.001) genotypes. Conclusion. These data suggested that the rs17860508 GC/GC genotype might upregulate IL-12B expression at the transcriptional level and thus increase the risk of CRC.
基金：
THis work was supported by a
grant from the National Natural Science Foundation of
China (no. 81372150) and by a grant from the Health and
Family Planning Commission of Hebei Province, China
(20170732).
语种：
外文
被引次数：
WOS：
PubmedID：
中科院分区：
出版当年[2020]版：
大类|3 区生物
小类|3 区生物工程与应用微生物4 区医学：研究与实验
最新[2025]版：
大类|4 区医学
小类|4 区生物工程与应用微生物4 区医学：研究与实验
JCR分区：
出版当年[2020]版：
Q2BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3MEDICINE, RESEARCH & EXPERIMENTAL
最新[2023]版：
Q3BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3MEDICINE, RESEARCH & EXPERIMENTAL
第一作者机构：[1]Department of General Surgery, Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang, Hebei 050011, China
通讯作者：
推荐引用方式(GB/T 7714)：
Liu Yabin,Li Binghui,Wang Lili,et al.A Functional Polymorphism in the Promoter Region of Interleukin-12B Increases the Risk of Colorectal Cancer[J].BIOMED RESEARCH INTERNATIONAL.2020,2020:doi:10.1155/2020/2091781.
APA：
Liu, Yabin,Li, Binghui,Wang, Lili&Kong, Dexian.(2020).A Functional Polymorphism in the Promoter Region of Interleukin-12B Increases the Risk of Colorectal Cancer.BIOMED RESEARCH INTERNATIONAL,2020,
MLA：
Liu, Yabin,et al."A Functional Polymorphism in the Promoter Region of Interleukin-12B Increases the Risk of Colorectal Cancer".BIOMED RESEARCH INTERNATIONAL 2020.(2020)
