机构：[1]College of Integrated Chinese and Western Medicine, Hebei Medical University, Shijiazhuang, Hebei 050017, China[2]Department of Traditional Chinese Medicine, Tumor Hospital of Hebei Province, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, China临床科室中医科河北医科大学第四医院[3]Department of Ecsomatics, Tangshan Maternal and Children Hospital, Tangshan, China
The present study is mainly to explore the mechanism that how Qigesan (QGS) affects the movement capacity of esophageal cancer (EC) cell. QGS incubates ECA109 and TE1 cell lines and detecting the motility of tumor cells by different experiments. Growth arrest-specific 6 (Gas6) and Anexelekto (Axl) were co-localized, and then detecting Gas6, Axl signaling pathway, and protein expression after QGS intervention. Similarly, Observing the signal localization and protein expression of P-phosphoinositide3-kinases (PI3K), P-AKT protein kinase B (AKT), P-nuclear factor-kappa B (NF-kappa B), matrixmetalloproteinase-2 (MMP2), and matrix metalloproteinase-9 (MMP9). The results showed that the concentration of QGS was less than 200 ug/ml, and the cultured cells did not exceed 24 h, that no obvious cytotoxicity was observed. QGS significantly inhibited the mobility of ECA109 and TE1 cell lines in the concentration-dependent manner. In addition, QGS can regulate the Gas6/Axl pathway, inhibit the formation and localization of the Gas6/Axl complex, and reduce the protein activation of PI3K/AKT, NF-kappa B, MMP2, and MMP9. Experimental innovation shows that QGS can significantly slow down the mobility of EC cells by regulating the Gas6/Axl complex and downstream signaling pathways, and provides a theoretical basis for the pharmacological effects of QGS in the therapy of EC.
基金：
Hebei dysphagia Key Laboratory of Chinese Medicine (Hebei, China); National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81403310]; Key Discipline Fund of College of Integrated Traditional Chinese and Western Medicine, Hebei Medical University
第一作者机构：[1]College of Integrated Chinese and Western Medicine, Hebei Medical University, Shijiazhuang, Hebei 050017, China
通讯作者：
通讯机构：[1]College of Integrated Chinese and Western Medicine, Hebei Medical University, Shijiazhuang, Hebei 050017, China[2]Department of Traditional Chinese Medicine, Tumor Hospital of Hebei Province, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, China
推荐引用方式(GB/T 7714)：
Kong Lingyu,Wu Zhongbing,Zhao Yang,et al.Qigesan reduces the motility of esophageal cancer cells via inhibiting Gas6/Axl and NF-kappa B expression[J].BIOSCIENCE REPORTS.2019,39:doi:10.1042/BSR20190850.
APA：
Kong, Lingyu,Wu, Zhongbing,Zhao, Yang,Lu, Xin,Shi, Huijuan...&Li, Jing.(2019).Qigesan reduces the motility of esophageal cancer cells via inhibiting Gas6/Axl and NF-kappa B expression.BIOSCIENCE REPORTS,39,
MLA：
Kong, Lingyu,et al."Qigesan reduces the motility of esophageal cancer cells via inhibiting Gas6/Axl and NF-kappa B expression".BIOSCIENCE REPORTS 39.(2019)
