机构：[1]Department of Surgery, Hebei Medical University, Shjjazhuang， China, 050017[2]Department of Functional Region of Diagnosis, Hebei Medical University Fourth Hospital, Hebei Medical University, Shijiazhuang, China, 050011医技科室功能科河北医科大学第四医院[3]Undergraduate, College of Basic Medicine, Hebei Medical University, Shijiazhuang, China, 050017[4]Department of Senile Disease, the Third Hospital of Hebei Medical University, Shijiazhuang，China, 050000[5]Molecular Hydrogen Foundation， Kissimmee FL, 34744，USA[6]Department of Biochemistry and Molecular Biology, Hebei Medical University，Shijiazhuang, China, 050017[7]Achievements Transformation Center, Hebei Medical University, Shijazhuang, China, 050017[8]Lingshui General Hospital, Hainan Province，China, 572400[9]Hebei Key Laboratory of Forensic Medicine, Hebei Medicinal University, China, 050017[10]Collaborative Innovation Center of Forensic Medical Molecular ldentification, Hebei Medicinal University, China, 050017
Due to its anti-inflammatory and anti-oxidative effects, recent research has demonstrated that molecular hydrogen can serve as a new medical approach for depression, anxiety and traumatic brain injury. However, its potential effects on neurodevelopmental diseases, such as autism are still elusive. The present study aims to investigate the potential effects of hydrogen-rich water (HRW) administration on valproic acid (VPA)-induced autistic-like behavioral deficits, and the associated underlying mechanism in adolescent mice offspring. Pregnant ICR mice were randomly divided into five groups (n = 6). One group was injected with saline (NAV group) and provided hydrogen-free water. The other four groups were injected with VPA (600 mg/kg, intraperitoneally, i.p.) on pregnant day (PND) 12.5. One group was provided with hydrogen-free water (VEH group) and the other three groups were provided HRW at different segments, postnatal day 1 (PND 1) to PND 21 (PHV group), PND 13 to PND 21 (PVS group) or from PND 13 to postnatal day 42 (PVL group). Behavioral tests, including open field, novelty suppressed feeding (NSF), hot plate, social interaction (SI) and contextual fear memory tests were conducted between postnatal day 35-42. We found that HRW administration significantly reversed the autistic-like behaviors induced by maternal VPA exposure in the adolescent offspring of both male and female adolescent offspring. Furthermore, HRW administration significantly reversed the alternation of serum levels of interleukin 6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha), but without any effects on the BDNF levels in maternal VPA-exposed mice offspring. These data suggest the need for additional research on HRW as a potential preventive strategy for autism and related disorders. Lay Summary: Maternal VPA injection induces autistic-like behavioral deficits in adolescent mice offspring. HRW administration ameliorates autistic-like behavioral deficits. HRW administration reverses the alternation of serum levels of IL-6 and TNF-alpha induced by VPA.
基金：
National Natural Science Foundation of China(81771462)，Natural Science Foundation from Hebei province， China
(No.2018206119)，Natural Science Foundation for Heath Science from HFPC of
Hebei Province (20170642, 20170470, 20170471), the College Students Innovative
Pilot Project in Hebei Medical University (USIP2016033, USIP2016035).
第一作者机构：[1]Department of Surgery, Hebei Medical University, Shjjazhuang， China, 050017
共同第一作者：
通讯作者：
通讯机构：[6]Department of Biochemistry and Molecular Biology, Hebei Medical University，Shijiazhuang, China, 050017[7]Achievements Transformation Center, Hebei Medical University, Shijazhuang, China, 050017[8]Lingshui General Hospital, Hainan Province，China, 572400[9]Hebei Key Laboratory of Forensic Medicine, Hebei Medicinal University, China, 050017[10]Collaborative Innovation Center of Forensic Medical Molecular ldentification, Hebei Medicinal University, China, 050017
推荐引用方式(GB/T 7714)：
Guo Qingjun,Yin Xi,Qiao Meng,et al.Hydrogen-Rich Water Ameliorates Autistic-Like Behavioral Abnormalities in Valproic Acid-Treated Adolescent Mice Offspring[J].FRONTIERS IN BEHAVIORAL NEUROSCIENCE.2018,12:doi:10.3389/fnbeh.2018.00170.
APA：
Guo, Qingjun,Yin, Xi,Qiao, Meng,Jia, Yujiao,Chen, Dandan...&Jia, Bin.(2018).Hydrogen-Rich Water Ameliorates Autistic-Like Behavioral Abnormalities in Valproic Acid-Treated Adolescent Mice Offspring.FRONTIERS IN BEHAVIORAL NEUROSCIENCE,12,
MLA：
Guo, Qingjun,et al."Hydrogen-Rich Water Ameliorates Autistic-Like Behavioral Abnormalities in Valproic Acid-Treated Adolescent Mice Offspring".FRONTIERS IN BEHAVIORAL NEUROSCIENCE 12.(2018)
