机构：[1]Hebei Med Univ, Hosp 4, Dept Clin Biocell, Shijiazhuang 050000, Hebei, Peoples R China河北医科大学第四医院[2]Hebei Gen Hosp, Dept Oncol, Shijiazhuang 050000, Hebei, Peoples R China[3]Zhejiang Chinese Med Univ, Clin Med Coll 3, Dept Neurobiol & Acupuncture Res, Hangzhou 310053, Zhejiang, Peoples R China[4]Hebei Gen Hosp, Dept Nursing, Shijiazhuang 050000, Hebei, Peoples R China[5]Hebei Med Univ, Hosp 4, Res Ctr, Shijiazhuang 050000, Hebei, Peoples R China河北医科大学第四医院[6]Hebei Gen Hosp, Dept Pathol, Shijiazhuang 050000, Hebei, Peoples R China[7]Zhejiang Chinese Med Univ, Lab & Equipment Adm, Hangzhou 310053, Zhejiang, Peoples R China
A murine monoclonal antibody (MAb-1) specific for GM3 has been generated by immunizing beta 3Gn-T5 knockout mice with purified GM3 ganglioside. The binding specificity of MAb-1 (IgG(3) subclass) was established by an enzyme-linked immunosorbent assay (ELISA) and FACS and the antibody showed high binding specificity with GM3. Cell viability assay showed that MAb-1 significantly suppressed cell growth. Immunohistochemistry analysis revealed that MAb-1 was strongly expressed in human ovarian cancer tissues, whereas it was hardly expressed in normal tissues. Finally, antibody-dependent cellular cytotoxicity (ADCC) activities were determined by measuring lactate dehydrogenase (LDH) releasing assay and the results showed high ADCC activities in two representative ovarian cancer cell lines (OVHM and ID8). All of these data indicate that MAb-1 may be potentially used as a therapeutic antibody against ovarian cancers in clinical trials.
基金：
National Natural Science Foundation of China (Grant No. 81301880 and 81603676),
Natural Science Foundation of Hebei Province (Grant No. H2015206418), Major Research Project of Hebei
Province (Grant No. zd2013006), projects from Health and Family Planning Commission of Hebei Province
(Grant No. 20130251 and 20140160), Zhejiang Provincial Natural Science Funds for Distinguished Young
Scholars (Grant No. LR17H270001) and Qianjiang Talent Program (Grant No.QJD1702020)
第一作者机构：[1]Hebei Med Univ, Hosp 4, Dept Clin Biocell, Shijiazhuang 050000, Hebei, Peoples R China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
He Dongwei,Fan Xiaoyan,Liu Boyi,et al.Generation and characterization of a IgG monoclonal antibody specific for GM3 (NeuGc) ganglioside by immunizing beta 3Gn-T5 knockout mice[J].SCIENTIFIC REPORTS.2018,8:doi:10.1038/s41598-018-20951-8.
APA：
He, Dongwei,Fan, Xiaoyan,Liu, Boyi,Tian, Yiqing,Zhang, Xiangmei...&Cai, Jianhui.(2018).Generation and characterization of a IgG monoclonal antibody specific for GM3 (NeuGc) ganglioside by immunizing beta 3Gn-T5 knockout mice.SCIENTIFIC REPORTS,8,
MLA：
He, Dongwei,et al."Generation and characterization of a IgG monoclonal antibody specific for GM3 (NeuGc) ganglioside by immunizing beta 3Gn-T5 knockout mice".SCIENTIFIC REPORTS 8.(2018)
