机构：[1]Department of Gynecology Ultrasound, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China河北医科大学第四医院[2]Department of Gastroenterology and Hepatology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China临床科室消化内科河北医科大学第四医院[3]Animal Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China河北医科大学第四医院[4]Department of Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China河北医科大学第四医院
BackgroundMicroRNA (miRNA)-related single nucleotide polymorphisms (miR-SNPs) in miRNA processing machinery genes are implicated in carcinogenesis, as they change the expression profiles of miRNA. Six miR-SNPs in miRNA processing machinery genes, including Dicer (rs3742330), RAN (rs14035), XPO5 (rs11077), TNRC6B (rs9623117), GEMIN3 (rs197412), and GEMIN4 (rs2740348), were evaluated for their association with esophageal squamous cell carcinoma (ESCC). MethodsThe miR-SNP of the miRNA processing genes were genotyped using the polymerase chain reaction-ligase detection reaction (PCR-LDR) assay, while the XPO5 expression levels in ESCC tissues were measured by immunochemistry methods. ResultsPatients carrying the rs11077 AA allele exhibited a significantly increased lifespan than AC+CC carriers, as determined by univariate and multivariate analyses (relative risk: 2.490; 95% confidence interval [CI]: 1.225-5.058; P=.012). Furthermore, the rs11077 AA genotype displayed a trend for high XPO5 expression in ESCC tissues by immunochemistry analysis, and these high XPO5 expression levels were also associated with high survival rates among ESCC patients. ConclusionOur results suggested that the miRNA machinery gene expression-associated miR-SNPs would modify cancer outcomes; in this light, XPO5 may be an important new target for ESCC therapy.
基金：
Key Basic Research Program of Hebei, Grant/
Award Number: 14967713D
第一作者机构：[1]Department of Gynecology Ultrasound, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China
通讯作者：
通讯机构：[3]Animal Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China[4]Department of Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China[*1]Department of Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China.[*2]Animal Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China.
推荐引用方式(GB/T 7714)：
Wang Cuiju,Dong Hailing,Fan Haiyan,et al.Genetic polymorphisms of microRNA machinery genes predict overall survival of esophageal squamous carcinoma[J].JOURNAL OF CLINICAL LABORATORY ANALYSIS.2018,32(1):doi:10.1002/jcla.22170.
APA：
Wang, Cuiju,Dong, Hailing,Fan, Haiyan,Wu, Jianhua&Wang, Guiying.(2018).Genetic polymorphisms of microRNA machinery genes predict overall survival of esophageal squamous carcinoma.JOURNAL OF CLINICAL LABORATORY ANALYSIS,32,(1)
MLA：
Wang, Cuiju,et al."Genetic polymorphisms of microRNA machinery genes predict overall survival of esophageal squamous carcinoma".JOURNAL OF CLINICAL LABORATORY ANALYSIS 32..1(2018)
