Comprehensive profiling and quantitation of oncogenic mutations in non-small cell lung carcinoma using single-molecule amplification and re-sequencing technology
机构：[1]Department of Medical Oncology, Fourth Hospital of Hebei Medical University, Shijiazhuang, China临床科室肿瘤内科河北医科大学第四医院[2]College of Science and Technology, China Three Gorges University, Yichang, China[3]Department of Pathology, Fourth Hospital of Hebei Medical University, Shijiazhuang, China医技科室病理科河北医科大学第四医院[4]Department of Medical Record Library, Fourth Hospital of Hebei Medical University, Shijiazhuang, China行政科室病案室河北医科大学第四医院
The carcinogenesis of non-small cell lung carcinoma has been found to associate with activating and resistant mutations in the tyrosine kinase domain of specific oncogenes. Here, we assessed the type, frequency, and abundance of epithelial growth factor receptor, KRAS, BRAF, and ALK mutations in 154 non-small cell lung carcinoma specimens using single-molecule amplification and re-sequencing technology. We found that epithelial growth factor receptor mutations were the most prevalent (44.2%), followed by KRAS (18.8%), ALK (7.8%), and BRAF (5.8%) mutations. The type and abundance of the mutations in tumor specimens appeared to be heterogeneous. Thus, we conclude that identification of clinically significant oncogenic mutations may improve the classification of patients and provide valuable information for determination of the therapeutic strategies.
第一作者机构：[1]Department of Medical Oncology, Fourth Hospital of Hebei Medical University, Shijiazhuang, China[*1]Department of Medical Oncology, Fourth Hospital of Hebei Medical University, No. 12, Jiankang Road, Shijiazhuang 050011, China.
通讯作者：
通讯机构：[1]Department of Medical Oncology, Fourth Hospital of Hebei Medical University, Shijiazhuang, China[*1]Department of Medical Oncology, Fourth Hospital of Hebei Medical University, No. 12, Jiankang Road, Shijiazhuang 050011, China.
推荐引用方式(GB/T 7714)：
Shi Jian,Yuan Meng,Wang Zhan-Dong,et al.Comprehensive profiling and quantitation of oncogenic mutations in non-small cell lung carcinoma using single-molecule amplification and re-sequencing technology[J].TUMOR BIOLOGY.2017,39(2):doi:10.1177/1010428317691413.
APA：
Shi, Jian,Yuan, Meng,Wang, Zhan-Dong,Xu, Xiao-Li,Hong, Lei&Sun, Shenglin.(2017).Comprehensive profiling and quantitation of oncogenic mutations in non-small cell lung carcinoma using single-molecule amplification and re-sequencing technology.TUMOR BIOLOGY,39,(2)
MLA：
Shi, Jian,et al."Comprehensive profiling and quantitation of oncogenic mutations in non-small cell lung carcinoma using single-molecule amplification and re-sequencing technology".TUMOR BIOLOGY 39..2(2017)
