Background/Aims: Periplocin is extracted from the traditional herbal medicine cortex periplocae, which has been reported to suppress the growth of cancer cells. However, little is known about its effect on gastric cancer cells. Methods: Gastric cancer cells were treated with periplocin, and cell viability was assessed using MIS assay. Flow cytometry and TUNEL staining were performed to evaluate apoptosis, and protein expression was examined by western blotting. Microarray analysis was used to screen for changes in related genes. Results: We found that periplocin had an inhibitory effect on gastric cancer cell viability in a dose-dependent manner. Periplocin inhibited cell viability via the ERK1/2-EGR1 pathway to induce apoptosis. Periplocin also inhibited the growth of tumor xenografts and induced apoptosis in vivo. Conclusion: Our results show that periplocin inhibits the proliferation of gastric cancer cells and induces apoptosis in vitro and in vivo, indicating its potential to be used as an antitumor drug. (C) 2016 The Author(s) Published by S. Karger AG, Basel
基金：
This study was supported by the National Natural Science Foundation of China (Grant
No.81502032, 81173611), Hebei Province Science and Technology Plan Project (Grant
No.H2015206376) and Financial department of Hebei Province (Grant No.20142065].
第一作者机构：[1]Research Centre, the Fourth Hospital of Hebei Medical University, Shijiazhuang, China
共同第一作者：
通讯作者：
通讯机构：[1]Research Centre, the Fourth Hospital of Hebei Medical University, Shijiazhuang, China[*1]Research Centre, the Fourth Hospital of Hebei Medical University, 12# Jiankang road, Shijiazhuang, 050011, (China)
推荐引用方式(GB/T 7714)：
Li Lei,Zhao Lian-Mei,Dai Su-li,et al.Periplocin Extracted from Cortex Periplocae Induced Apoptosis of Gastric Cancer Cells via the ERK1/2-EGR1 Pathway[J].CELLULAR PHYSIOLOGY AND BIOCHEMISTRY.2016,38(5):1939-1951.doi:10.1159/000445555.
APA：
Li, Lei,Zhao, Lian-Mei,Dai, Su-li,Cui, Wen-Xuan,Lv, Hui-Lai...&Shan, Bao-En.(2016).Periplocin Extracted from Cortex Periplocae Induced Apoptosis of Gastric Cancer Cells via the ERK1/2-EGR1 Pathway.CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,38,(5)
MLA：
Li, Lei,et al."Periplocin Extracted from Cortex Periplocae Induced Apoptosis of Gastric Cancer Cells via the ERK1/2-EGR1 Pathway".CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 38..5(2016):1939-1951
