高级检索
当前位置: 首页 > 详情页

Determination of salvianolic acid C in rat plasma using liquid chromatography-mass spectrometry and its application to pharmacokinetic study

文献详情

资源类型:
WOS体系:
Pubmed体系:

收录情况: ◇ SCIE

机构: [1]State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Beijing Key Laboratory of Drug Target Identification and Drug Screening, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, China [2]Pharmacy Department of the Affiliated Hospital of Qingdao University, Qingdao, 266003, China [3]The Fourth Hospital of Hebei Medical University, Shijiazhuang, 050011, China
出处:
ISSN:

关键词: salvianolic acid C LC-MS pharmacokinetics bioavailability dose proportionality

摘要:
A sensitive and reliable LC-ESI-MS method for the determination of salvianolic acid C in rat plasma has been developed and validated. Plasma samples were prepared by liquid-liquid extraction with ethyl acetate and separated on a Zorbax SB-C-18 column (3.5 mu m, 2.1x100mm) at a flow rate of 0.3mL/min using acetonitrile-water as mobile phase. The detection was carried out by a single quadrupole mass spectrometer with electrospray ionization source and selected ion monitoring mode. Linearity was obtained for salvianolic acid C ranging from 5 to 1000ng/mL. The intra- and inter-day precisions (RSD, %) didn't exceed 9.96%, and the accuracy (RE, %) were all within +/- 3.64%. The average recoveries of the analyte and internal standard were >89.13%. Salvianolic acid C was proved to be stable during all sample storage, preparation and analytic procedures. The validated method was successfully applied to pharmacokinetic study after oral and intravenous administration of salvianolic acid C to rats. The absolute oral bioavailability of salvianolic acid C was 0.29 +/- 0.05%. This method was further applied to simultaneous determination of salvianolic acid A, salvianolic acid B and salvianolic acid C in rat plasma and showed good practicability. Copyright (c) 2015 John Wiley & Sons, Ltd.

基金:
语种:
被引次数:
WOS:
PubmedID:
中科院分区:
出版当年[2016]版:
大类 | 4 区 生物
小类 | 4 区 生化研究方法 4 区 生化与分子生物学 4 区 分析化学 4 区 药学
最新[2025]版:
大类 | 4 区 医学
小类 | 4 区 生化研究方法 4 区 生化与分子生物学 4 区 分析化学 4 区 药学
JCR分区:
出版当年[2016]版:
Q3 CHEMISTRY, ANALYTICAL Q4 BIOCHEMICAL RESEARCH METHODS Q4 PHARMACOLOGY & PHARMACY Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
最新[2024]版:
Q3 CHEMISTRY, ANALYTICAL Q3 PHARMACOLOGY & PHARMACY Q4 BIOCHEMICAL RESEARCH METHODS Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

影响因子: 最新[2024版] 最新五年平均 出版当年[2016版] 出版当年五年平均 出版前一年[2015版] 出版后一年[2017版]

第一作者:
第一作者机构: [1]State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Beijing Key Laboratory of Drug Target Identification and Drug Screening, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, China
通讯作者:
通讯机构: [1]State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Beijing Key Laboratory of Drug Target Identification and Drug Screening, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, China [*1]State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Beijing Key Laboratory of Drug Target Identification and Drug Screening, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, China.
推荐引用方式(GB/T 7714):
APA:
MLA:

资源点击量:42313 今日访问量:0 总访问量:1365 更新日期:2025-08-01 建议使用谷歌、火狐浏览器 常见问题

技术支持:重庆聚合科技有限公司 地址:河北省石家庄市健康路12号