机构：[1]State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Beijing Key Laboratory of Drug Target Identification and Drug Screening, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, China[2]Pharmacy Department of the Affiliated Hospital of Qingdao University, Qingdao, 266003, China[3]The Fourth Hospital of Hebei Medical University, Shijiazhuang, 050011, China河北医科大学第四医院临床药理研究部药学部
A sensitive and reliable LC-ESI-MS method for the determination of salvianolic acid C in rat plasma has been developed and validated. Plasma samples were prepared by liquid-liquid extraction with ethyl acetate and separated on a Zorbax SB-C-18 column (3.5 mu m, 2.1x100mm) at a flow rate of 0.3mL/min using acetonitrile-water as mobile phase. The detection was carried out by a single quadrupole mass spectrometer with electrospray ionization source and selected ion monitoring mode. Linearity was obtained for salvianolic acid C ranging from 5 to 1000ng/mL. The intra- and inter-day precisions (RSD, %) didn't exceed 9.96%, and the accuracy (RE, %) were all within +/- 3.64%. The average recoveries of the analyte and internal standard were >89.13%. Salvianolic acid C was proved to be stable during all sample storage, preparation and analytic procedures. The validated method was successfully applied to pharmacokinetic study after oral and intravenous administration of salvianolic acid C to rats. The absolute oral bioavailability of salvianolic acid C was 0.29 +/- 0.05%. This method was further applied to simultaneous determination of salvianolic acid A, salvianolic acid B and salvianolic acid C in rat plasma and showed good practicability. Copyright (c) 2015 John Wiley & Sons, Ltd.
基金：
Key Project for Drug
Innovation (no. 2009ZX09102-123), National Natural Science Foundation
of China (no. 81102492) and Major Scientific and
Technological Special Project for ‘Significant New Drugs Creation’
(no. 2012ZX09301002001001 and 2013ZX09508104001002).
语种：
外文
被引次数：
WOS：
PubmedID：
中科院分区：
出版当年[2016]版：
大类|4 区生物
小类|4 区生化研究方法4 区生化与分子生物学4 区分析化学4 区药学
最新[2025]版：
大类|4 区医学
小类|4 区生化研究方法4 区生化与分子生物学4 区分析化学4 区药学
JCR分区：
出版当年[2016]版：
Q3CHEMISTRY, ANALYTICALQ4BIOCHEMICAL RESEARCH METHODSQ4PHARMACOLOGY & PHARMACYQ4BIOCHEMISTRY & MOLECULAR BIOLOGY
最新[2024]版：
Q3CHEMISTRY, ANALYTICALQ3PHARMACOLOGY & PHARMACYQ4BIOCHEMICAL RESEARCH METHODSQ4BIOCHEMISTRY & MOLECULAR BIOLOGY
第一作者机构：[1]State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Beijing Key Laboratory of Drug Target Identification and Drug Screening, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, China
通讯作者：
通讯机构：[1]State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Beijing Key Laboratory of Drug Target Identification and Drug Screening, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, China[*1]State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Beijing Key Laboratory of Drug Target Identification and Drug Screening, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, China.
推荐引用方式(GB/T 7714)：
Song Junke,Zhang Wen,Sun Jialin,et al.Determination of salvianolic acid C in rat plasma using liquid chromatography-mass spectrometry and its application to pharmacokinetic study[J].BIOMEDICAL CHROMATOGRAPHY.2016,30(3):376-383.doi:10.1002/bmc.3558.
APA：
Song, Junke,Zhang, Wen,Sun, Jialin,Zhang, Xue,Xu, Xiaona...&Du, Guanhua.(2016).Determination of salvianolic acid C in rat plasma using liquid chromatography-mass spectrometry and its application to pharmacokinetic study.BIOMEDICAL CHROMATOGRAPHY,30,(3)
MLA：
Song, Junke,et al."Determination of salvianolic acid C in rat plasma using liquid chromatography-mass spectrometry and its application to pharmacokinetic study".BIOMEDICAL CHROMATOGRAPHY 30..3(2016):376-383
