机构：[1]Research Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011河北医科大学第四医院[2]State Key Laboratory Breeding Base-Hebei Province Key Laboratory of Molecular Chemistry for Drug, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018[3]Breast Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China河北医科大学第四医院
Sansalvamide A, a cyclic depsipeptide isolated from a marine fungus of the Fusarium genus, exhibits significant antitumor activity. In the present study, H-10 (molecular formula, C38H55N5O6; molecular weight, 677.8732), a novel sansalvamide A derivative, demonstrated an inhibitory effect on the proliferation of murine melanoma B16 cells. It was confirmed that H-10 induced the apoptosis of the B16 cells. The inhibitory rate of various concentrations of H-10 on the B16 cells was measured by sulforhodamine B colorimetric assay, and the results revealed that the inhibitory effect of H-10 on the B16 cells occurred in a concentration-dependent manner. In addition, a growth curve model of the B16 cells treated with 50 mu M H-10 revealed that the effect of H-10 also occurred in a time-dependent manner. The apoptotic morphology of the B16 cells was observed using an optical microscope. Following the treatment of the cells with 50 mu M H-10 for 24 h, the cell apoptosis rate was analyzed using flow cytometry. The expression levels of caspase-3, -8 and -9 were analyzed by western blot analysis, and the results indicated that H-10 may induce the apoptosis of B16 cells.
基金：
National Basic Research Program of ChinaNational Basic Research Program of China [2011CB512007, 2012CB723501]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30873139]
第一作者机构：[1]Research Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011
通讯作者：
通讯机构：[1]Research Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011[*1]Research Center, The Fourth Hospital of Hebei Medical University, 12 Jiankang Road, Shijiazhuang, Hebei 050011, P.R. China
推荐引用方式(GB/T 7714)：
Zhang Geng,Liu Shouxin,Liu Yunjiang,et al.A novel cyclic pentapeptide, H-10, inhibits B16 cancer cell growth and induces cell apoptosis[J].ONCOLOGY LETTERS.2014,8(1):248-252.doi:10.3892/ol.2014.2121.
