Decreased miRNA-148a is associated with lymph node metastasis and poor clinical outcomes and functions as a suppressor of tumor metastasis in non-small cell lung cancer
机构：[1]Department of Medical Oncology, Subei People's Hospital, Clinical Medical College of Yangzhou University, Yangzhou, Jiangsu 225001[2]Department of Respiratory Medicine , Subei People's Hospital, Clinical Medical College of Yangzhou University, Yangzhou, Jiangsu 225001[3]Department of Gynecology and Obstetrics, Subei People's Hospital, Clinical Medical College of Yangzhou University, Yangzhou, Jiangsu 225001[4]Department of Medical Oncology, Hebei Medical University Fourth Hospital, Shijiazhuang, Hebei 050011临床科室肿瘤内科河北医科大学第四医院[5]Department of Medical Oncology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, Jiangsu 210002[6]Department of Pathology, Subei People's Hospital, Clinical Medical College of Yangzhou University, Yangzhou, Jiangsu 225001[7]Department of Cytobiology, School of Medicine, Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China
Non-small cell lung cancer (NSCLC) is the leading cause of cancer-related death worldwide, and only 15% of lung cancer patients live more than 5 years. microRNAs (miRNAs) are endogenously expressed non-coding RNAs, and dysregulation of miRNAs is a common feature in human cancers including lung cancer. In this study, we describe the epigenetic regulation of miRNA-148a and its prognostic value in NSCLC. Due to hypermethylation of the miRNA-148a encoding region, the expression levels of miRNA-148a were decreased in NSCLC tissues and cells. Decreased miRNA-148a expression was associated with lymph node metastasis, advanced clinical stage and shortened disease-free survival and overall survival in NSCLC, and was an independent prognostic factor for overall survival in multivariate analysis. In vitro, overexpression of miRNA-148a significantly suppressed the migratory and invasive abilities of A549 and H1299 lung cancer cells. Enforced expression of miRNA-148a in lung cancer cell lines resulted in a significant reduction in the expression of DNMT1. This, in turn, led to a decrease in DNA methylation of the tumor-suppressor gene E-cadherin and induced an increase in the protein levels of E-cadherin. By understanding the function and molecular mechanism of miRNA-148a in NSCLC, miRNA-148a may have therapeutic potential to suppress lung cancer metastasis.
第一作者机构：[1]Department of Medical Oncology, Subei People's Hospital, Clinical Medical College of Yangzhou University, Yangzhou, Jiangsu 225001
共同第一作者：
通讯作者：
通讯机构：[2]Department of Respiratory Medicine , Subei People's Hospital, Clinical Medical College of Yangzhou University, Yangzhou, Jiangsu 225001[5]Department of Medical Oncology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, Jiangsu 210002[*1]Department of Medical Oncology, Jinling Hospital, School of Medicine, Nanjing University, 140 Hanzhong Road, Nanjing, Jiangsu 210002, P.R. China[*2]Department of Respiratory Medicine, Subei People's Hospital, Clinical Medical College of Yangzhou University, No. 98 Nantong West Road, Yangzhou, Jiangsu 225001, P.R. China
推荐引用方式(GB/T 7714)：
Chen Yong,Min Lingfeng,Zhang Xizhi,et al.Decreased miRNA-148a is associated with lymph node metastasis and poor clinical outcomes and functions as a suppressor of tumor metastasis in non-small cell lung cancer[J].ONCOLOGY REPORTS.2013,30(4):1832-1840.doi:10.3892/or.2013.2611.
APA：
Chen, Yong,Min, Lingfeng,Zhang, Xizhi,Hu, Suwei,Wang, Buhai...&Chen, Longbang.(2013).Decreased miRNA-148a is associated with lymph node metastasis and poor clinical outcomes and functions as a suppressor of tumor metastasis in non-small cell lung cancer.ONCOLOGY REPORTS,30,(4)
MLA：
Chen, Yong,et al."Decreased miRNA-148a is associated with lymph node metastasis and poor clinical outcomes and functions as a suppressor of tumor metastasis in non-small cell lung cancer".ONCOLOGY REPORTS 30..4(2013):1832-1840
