机构：[1]Department of Thoracic Surgery, Fourth Hospital, Hebei Medical University, Shiziazhuang, China河北医科大学第四医院[2]Department of Surgery, Royal Adelaide Hospital, Adelaide University, Adelaide, Australia[3]Department of Endocrinology ,Fourth Hospital,Hebei Medical University, Shijiazhuang, China临床科室内分泌科河北医科大学第四医院[4]Scientific Research Centre,Fourth Hospital,Hebei Medical University, Shijiazhuang, China医技科室科研中心河北医科大学第四医院[5]School of Nursing and Midwifery, Flinders University, Adelaide, Australia
Although non-steroidal anti-inflammatory drugs (NSAIDs) have been demonstrated to have cancer-preventive effects and induce apoptosis of cancer cells, the mechanism of their effects is not clearly known. We studied the mechanism in human esophageal cancer cell line TE13. The esophageal squamous cell carcinoma cell line TE-13 was cultured with NS-398 at different concentrations or for different times. Proliferation and apoptosis were measured by MTT reduction and flow cytometry. Prostaglandin F1 was determined with radioimmunoassay. Expression of COX-2 mRNA was measured by RT-PCR and COX-2 protein levels with Western blot analysis. Nuclear NF-kappaB and cytoplasmic IkappaB protein levels were determined by electrophoretic mobility shift assay and Western blot, respectively. NS-398 significantly inhibited cell proliferation and induced apoptosis at concentrations of 0.001, 0.01, 1, and 100 mol/L. NS-398 dose-dependently decreased the levels of COX-2 mRNA, COX-2 protein, nuclear NF-kappaB protein and production of PGF1 and increased the cytoplasmic IkappaB protein. In conclusion, NS-398 inhibits the proliferation of, and induced apoptosis in, the cultured TE-13 SCC cell line. These changes correlate with a reduction in COX-2 mRNA and protein expression, prostaglandin synthesis, an inhibition of NF-kappaB nuclear translocation, and an increase in cytoplasmic IkappaB.
基金：
University of Adelaide Department of Surgery; Royal Adelaide Hospital; Hebei Provincial Natural Science Fund
第一作者机构：[1]Department of Thoracic Surgery, Fourth Hospital, Hebei Medical University, Shiziazhuang, China[*1]Department of Thoracic Surgery Fourth Hospital Hebei Medical University 12 Jiankang Road Shijiazhuang 050011 China
通讯作者：
通讯机构：[1]Department of Thoracic Surgery, Fourth Hospital, Hebei Medical University, Shiziazhuang, China[*1]Department of Thoracic Surgery Fourth Hospital Hebei Medical University 12 Jiankang Road Shijiazhuang 050011 China
推荐引用方式(GB/T 7714)：
Liu Jun Feng,Zhu Gui Jun,Jamieson Glyn G.,et al.NS-398 Induces Apoptosis in Human Esophageal Cancer Cells Through Inhibition of NF-kappaB Downstream Regulation of Cyclooxygenase-2[J].CANCER INVESTIGATION.2009,27(1):17-23.doi:10.1080/07357900801992913.
APA：
Liu, Jun Feng,Zhu, Gui Jun,Jamieson, Glyn G.,Wu, Tie Cheng,Zhu, Tie Nian...&Drew, Paul A..(2009).NS-398 Induces Apoptosis in Human Esophageal Cancer Cells Through Inhibition of NF-kappaB Downstream Regulation of Cyclooxygenase-2.CANCER INVESTIGATION,27,(1)
MLA：
Liu, Jun Feng,et al."NS-398 Induces Apoptosis in Human Esophageal Cancer Cells Through Inhibition of NF-kappaB Downstream Regulation of Cyclooxygenase-2".CANCER INVESTIGATION 27..1(2009):17-23
