机构：[1]Department of Women’s and Children’s Health, Section for Obstetrics and Gynecology, Uppsala University, 751 85 Uppsala[2]Ludwig Institute for Cancer Research, 751 24 Uppsala[3]Department of Obstetrics and Gynecology, 901 85 Umeå University, Sweden[4]Department of Obstetrics and Gynecology, the 4th Hospital of Hebei Medical University, 050011 Shijiazhuang, China临床科室产科河北医科大学第四医院
Background: It has been demonstrated that 2-Methoxyestradiol (2-ME), one of the estrogen metabolites, induces apoptosis in many different tumor cell lines. In the present study, the effects of 2-ME on human cervical cancer HeLaS3 cells and on normal cervical epithelial cells were evaluated. Materials and Methods: Acridine orange staining, DNA fragmentation arrays and flow cytometry were used to measure the apoptosis and cell cycle progression. In addition, the effect of 2-ME on expression of iNOS was measured by Western blot. Results: 2-ME inhibited the growth of HeLaS3 cells. This growth inhibition was accompanied by apoptosis and G(2)/M cell cycle arrest. 2-ME increased the expression of iNOS in parallel with apoptosis. Moreover, apoptosis was prevented by the iNOS inhibitor 1400W. 2-ME treatment resulted in a slight increase of the G(2)/M population, but no apoptosis, in normal cervical epithelial cells. There was no synergetic effect between E? and 2-ME. Conclusion: 2-ME induced apoptosis via the iNOS pathway and caused G(2)/M cell cycle arrest in human cervical cancer HeLaS3 cells, but showed only slight effects on normal cervical epithelial cells. These data suggest that 2-ME might be an adjuvant agent in the treatment of cervical cancer.
基金：
Swedish Medical
Research Council, Selanders fund and Family planning’s fund in
Uppsala, Sweden
第一作者机构：[1]Department of Women’s and Children’s Health, Section for Obstetrics and Gynecology, Uppsala University, 751 85 Uppsala[4]Department of Obstetrics and Gynecology, the 4th Hospital of Hebei Medical University, 050011 Shijiazhuang, China
通讯作者：
通讯机构：[1]Department of Women’s and Children’s Health, Section for Obstetrics and Gynecology, Uppsala University, 751 85 Uppsala[*1]Uppsala University, Section for Women’s and Children’s Health, Obstetrics and Gynecology, 751 85 Uppsala, Sweden
推荐引用方式(GB/T 7714)：
Li L,Bu SZ,Backstrom T,et al.Induction of apoptosis and G2/M arrest by 2-Methoxyestradiol in human cervical cancer HeLaS3 cells[J].ANTICANCER RESEARCH.2004,24(2B):873-880.
APA：
Li, L,Bu, SZ,Backstrom, T,Landstrom, M,Ulmsten, U&Fu, X.(2004).Induction of apoptosis and G2/M arrest by 2-Methoxyestradiol in human cervical cancer HeLaS3 cells.ANTICANCER RESEARCH,24,(2B)
MLA：
Li, L,et al."Induction of apoptosis and G2/M arrest by 2-Methoxyestradiol in human cervical cancer HeLaS3 cells".ANTICANCER RESEARCH 24..2B(2004):873-880
