机构：[1]Internal Medicine, Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang, China河北医科大学第四医院内分泌科临床科室[2]Environmental Health, Faculty of Medicine, University of Yamanashi, Tamaho, Yamanashi, Japan[3]Pathology II, Faculty of Medicine, University of Yamanashi, Tamaho, Yamanashi, Japan[4]Industrial Health Promotion Center of Yamanashi, Kofu, Japan
The present study was performed to determine the effect of long-term feeding with a high-fat/low-carbohydrate (HF/LC) diet on the onset of type-2 diabetes mellitus in normal rats. Male Wistar lmamichi rats were kept on a Control (carbohydrate 60%, fat 15%) or HF/ LC (carbohydrate 10%, fat 65%) diet for 16 mo. An intraperitoneal glucose tolerance test was performed once every 2 mo. Glucose tolerance was impaired 2 mo after the start of HF/LC diet feeding, accompanied by a decrease in the insulinogenic index. Along with time of HF/LC diet feeding, the glucose tolerance was further deteriorated with more serious impairment of insulin secretion and sensitivity. At the end of the experiment, 15 of 18 rats in the HF/LC group were diabetic, whereas only 4 of 17 rats in the Control group were diabetic. The present results demonstrate that long-term feeding with a HF/LC diet decreases the secretion and sensitivity of insulin, and induces diabetes mellitus in rats. Furthermore, long-term feeding with such a diet may produce adverse effects on the blood plasma lipid profile, with elevated levels of triglycerides, nonesterified fatty acids, total cholesterol, and reduced levels of high density protein cholesterol in the plasma.
基金：
grant-in-aid (12670347)
from the Ministry of Education, Science, Sports and Culture
of Japan
第一作者机构：[1]Internal Medicine, Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang, China
通讯作者：
通讯机构：[2]Environmental Health, Faculty of Medicine, University of Yamanashi, Tamaho, Yamanashi, Japan[*1]Department of Environmental Health, Faculty of Medicine, University of Yamanashi, Tamaho, Yamanashi, 409-3898 Japan
推荐引用方式(GB/T 7714)：
Wang Y,Wang PY,Qin LQ,et al.The development of diabetes mellitus in Wistar rats kept on a high-fat/low-carbohydrate diet for long periods[J].ENDOCRINE.2003,22(2):85-92.doi:10.1385/ENDO:22:2:85.
APA：
Wang, Y,Wang, PY,Qin, LQ,Davaasambuu, G,Kaneko, T...&Sato, A.(2003).The development of diabetes mellitus in Wistar rats kept on a high-fat/low-carbohydrate diet for long periods.ENDOCRINE,22,(2)
MLA：
Wang, Y,et al."The development of diabetes mellitus in Wistar rats kept on a high-fat/low-carbohydrate diet for long periods".ENDOCRINE 22..2(2003):85-92
