机构：[1]Department of Orthopedics, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei, People's Republic of China.河北医科大学第四医院[2]Department of Pathology, St Jude Children's Research Hospital, Memphis, TN 38105, USA.[3]Department of Computational Biology, St Jude Children's Research Hospital, Memphis, TN 38105, USA.[4]Department of Surgery, St Jude Children's Research Hospital, Memphis, TN 38105, USA.
Osteosarcoma (OS) cell lines are widely used in understanding the biological functions of cancer, identification and validation of therapeutic targets, as well as in vitro or in vivo preclinical drug screening. Here we report there is a frequent loss-of-function of polycomb repressive complex 2 (PRC2) in OS cell lines but it is rare in tumor samples based on genomic sequencing data, western blotting and immunohistochemistry analysis of H3K27me3. U2OS and 143B cell lines have a complete loss of function of PRC2 and several others have partial loss. In OS tumor tissues, only 1 out of 14 has low expression of H3K27me3. Kaplan-Meier analysis indicates that high EZH2, the component of PRC2, is associated with poor metastasis-free survival. Our observations are to raise the alarm that particular caution should be taken when using OS cell line models to study the disease, functional genomics, therapeutic target validation, drug screening, and epigenetic studies. Nevertheless, these cell lines will become useful biological tools to dissect the functions of PRC2.
基金：
American Cancer Society-Research Scholar (J.Y., 130421-RSG-17-071- 01-TBG), the National Cancer Institute (J.Y., R03 CA212802-01A1), the Assisi Foundation of Memphis, the American Lebanese Syrian Associated Charities (ALSAC), the US Public Health Service Childhood Solid Tumor Program Project Grant No. CA23099, the Cancer Center Support Grant No. 21766 from the National Cancer Institute, and the Key Project of Medical-Science Research of Hebei Province (HF, 20160159).
语种：
外文
PubmedID：
第一作者：
第一作者机构：[1]Department of Orthopedics, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei, People's Republic of China.
共同第一作者：
通讯作者：
通讯机构：[4]Department of Surgery, St Jude Children's Research Hospital, Memphis, TN 38105, USA.
推荐引用方式(GB/T 7714)：
Feng Helin,Tillman Heather,Wu Gang,et al.Frequent epigenetic alterations in polycomb repressive complex 2 in osteosarcoma cell lines.[J].Oncotarget.2018,9(43):27087-27091.doi:10.18632/oncotarget.25484.
APA：
Feng Helin,Tillman Heather,Wu Gang,Davidoff Andrew M&Yang Jun.(2018).Frequent epigenetic alterations in polycomb repressive complex 2 in osteosarcoma cell lines..Oncotarget,9,(43)
MLA：
Feng Helin,et al."Frequent epigenetic alterations in polycomb repressive complex 2 in osteosarcoma cell lines.".Oncotarget 9..43(2018):27087-27091
