Long non-coding RNA (lncRNA) PGM5P4-AS1 inhibits lung cancer progression by up-regulating leucine zipper tumor suppressor (LZTS3) through sponging microRNA miR-1275.
机构：[1]Department of Thoracic Surgery, The Fourth Hospital of Hebei Medical University , Shijiazhuang, P.R. China.河北医科大学第四医院[2]Department of Thoracic Surgery, Hebei Chest Hospital , Shijiazhuang, P.R. China.[3]Department of Thoracic Surgery, Hebei General Hospital , Shijiazhuang, P.R. China.
It is necessary to explore new molecules for the improvement of precise diagnosis and antitumor therapies in lung cancer. LncRNAs (long non-coding RNAs) play an important role in the regulation of cancer cell malignant behavior and tumor development. In this work, we found that a newly discovered lncRNA, lncRNA PGM5P4-AS1, was lower expressed in lung cancer tissues than adjacent tissues. Then, the lncRNA PGM5P4-AS1 was overexpressed or knocked-down in different lung cancer cells, and its effects on the malignant phenotypes were measured by 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, cell cycle assay, wound healing assay, and transwell assay. The results showed that the overexpression of PGM5P4-AS1 inhibited lung cancer cell proliferation, migration, and invasion activities, while these abilities were prominently promoted by the interference of PGM5P4-AS1. Further, the growth of lung cancer tumors in nude mice was also inhibited by PGM5P4-AS1 overexpression. In mechanism, PGM5P4-AS1 has the binding site of miR-1275 and could positively regulate the expression of LZTS3 via sponging miR-1275. In conclusion, PGM5P4-AS1 could be a potential precise diagnosis and therapeutic target biomarker of lung cancer.
基金：
This research was supported by grants from the Key Project of Health Technology Research and Achievement Conversion in Hebei Province (Grant Number zh2018009) and the Technological Innovation Project of Cooperation Fund in Hebei Provincial Department of Science and Technology and Hebei Medical University (Grant Number 2020TXZH04).
第一作者机构：[1]Department of Thoracic Surgery, The Fourth Hospital of Hebei Medical University , Shijiazhuang, P.R. China.[2]Department of Thoracic Surgery, Hebei Chest Hospital , Shijiazhuang, P.R. China.
通讯作者：
通讯机构：[1]Department of Thoracic Surgery, The Fourth Hospital of Hebei Medical University , Shijiazhuang, P.R. China.[*1]Department of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, , Shijiazhuang, Hebei 050011, P.R. China
推荐引用方式(GB/T 7714)：
Feng Junpeng,Li Jianhang,Qie Peng,et al.Long non-coding RNA (lncRNA) PGM5P4-AS1 inhibits lung cancer progression by up-regulating leucine zipper tumor suppressor (LZTS3) through sponging microRNA miR-1275.[J].BIOENGINEERED.2021,12(1):196-207.doi:10.1080/21655979.2020.1860492.
APA：
Feng Junpeng,Li Jianhang,Qie Peng,Li Zhenhua,Xu Yanzhao&Tian Ziqiang.(2021).Long non-coding RNA (lncRNA) PGM5P4-AS1 inhibits lung cancer progression by up-regulating leucine zipper tumor suppressor (LZTS3) through sponging microRNA miR-1275..BIOENGINEERED,12,(1)
MLA：
Feng Junpeng,et al."Long non-coding RNA (lncRNA) PGM5P4-AS1 inhibits lung cancer progression by up-regulating leucine zipper tumor suppressor (LZTS3) through sponging microRNA miR-1275.".BIOENGINEERED 12..1(2021):196-207
