Our study explores miR-150's effect on the biological activity of breast cancer cells and its correlation with Notch signaling. Human breast cancer cells MCF-7 were divided into WZ group (MCF-7 cells); KZ group (transfected with miR-150-NC); and group II (transfected with miR-150inhibitor) followed by analysis of miR-150 expressio,n cell replication, apoptosis, invasion, migration ability and Notch1 and Notch3 expression by qRT-PCR, cloning, Hoechst33258 fluorescent staining, Transwell chamber, cell scratch test, dual luciferase report and Western blot. Lowest miR-150 expression in MCF-7 cells indicated a successful transfection (P < 0.05). Compared with KZ and WZ groups, Notch1 and Notch3 mRNA levels in group II were decreased (P < 0.05); and the number of cell clones in group II was reduced (p< 0.05) without difference between WZ and KZ group (P > 0.05); miR-150 inhibitor reduced Notch1 and Notch3 expression (P < 0.05). The fluorescence intensity of MCF-7 cells in group II was highest among three groups (P < 0.05). The number of cell invasion and migration as well as Notch1 and Notch3 expression in group II was reduced ( P < 0.05) without difference between group KZ and WZ ( P > 0.05). miR-150 expression is increased in MCF-7 cells. The miR-150 inhibitor can inhibit cell apoptosis, migration and other biological behaviors, which is related to target Notch signaling pathway.