机构：[1]Department of Periodontics & Oral Mucosal Diseases, The Affiliated Stomatology Hospital of Southwest Medical University, Luzhou, Sichuan, China[2]Oral & Maxillofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, Luzhou, Sichuan, China[3]Department of Stomatology Technology, School of Medical Technology, Sichuan College of Traditional Medcine, Mianyang, China[4]Department of Orthodontics, Mianyang Stomatological Hospital, Mianyang, China[5]Department of Oral and Maxillofacial Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China临床科室口腔科河北医科大学第四医院[6]Department of Oral and Maxillofacial Surgery, The Second Hospital of Hebei Medical University, Shijiazhuang, China[7]Department of Oral Pathology, School and Hospital of Stomatology, Hebei Medical University & Hebei Key Laboratory of Stomatology, Shijiazhuang, China
Our study aimed to explore potential new diagnostic biomarkers in patients with oral squamous cell carcinoma (OSCC) to find new target molecules involved in the progression of OSCC. Potential novel biomarkers of OSCC were identified using a protein microarray assay. Compared with the healthy control group, there were five proteins (I309, GDF15, AXL, MMP3, and CTACK) in the serum of in situ oral cancer group. However, there were four differentially expressed proteins (MCSF, I309, MMP3, and CTACK) in the serum of the OSCC group. Receiver operating characteristic (ROC) curve analysis results suggested that these six proteins (I309, GDF15, AXL, MMP3, CTACK, and MCSF) had diagnostic value for OSCC. Based on The Cancer Genome Atlas (TCGA) database, we found that only GDF15 expression was associated with the prognosis of OSCC. Subsequently, we verified the expression levels of six proteins in HSC-3 and HaCaT cells, and the results showed that the level of these six proteins was significantly higher in HSC-3 cells than in normal HaCaT cells. Similarly, in the OSCC nude mouse model, the expression levels of these proteins were significantly upregulated in OSCC tumor tissue compared to the normal tissue. GDF-15, MMP-3, AXL, MCSF, I309, and CTACK may be used as biomarkers for OSCC diagnosis and provide a novel study direction for the treatment of OSCC.
基金：
The present study was supported by grants from Sichuan
Provincial Science and Technology Department Science and
Technology Project (no. 2020YJ0387), The Science and
Technology Strategic Cooperation Programs of Luzhou
Municipal People’s Government and Southwest Medical
University (no. 2019LZXNYDZ10 and 2020LZXNYDZ01)
and Luzhou Municipal Bureau of Science, Technology and
Intellectual Property, Sichuan Province (no. 2019-JYJ-54)
第一作者机构：[1]Department of Periodontics & Oral Mucosal Diseases, The Affiliated Stomatology Hospital of Southwest Medical University, Luzhou, Sichuan, China[2]Oral & Maxillofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, Luzhou, Sichuan, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Periodontics & Oral Mucosal Diseases, The Affiliated Stomatology Hospital of Southwest Medical University, Luzhou, Sichuan, China[2]Oral & Maxillofacial Reconstruction and Regeneration Laboratory, Southwest Medical University, Luzhou, Sichuan, China[*1]Department of Periodontics & Oral Mucosal Diseases, The Affiliated Stomatology Hospital of Southwest Medical University, 2 Jiangyang South Street, Luzhou, Sichuan 646000, P.R. China
推荐引用方式(GB/T 7714)：
Zhang Mengxue,Chen Xiao,Chen He,et al.Identification and validation of potential novel biomarkers for oral squamous cell carcinoma.[J].BIOENGINEERED.2021,12(1):8845-8862.doi:10.1080/21655979.2021.1987089.
APA：
Zhang Mengxue,Chen Xiao,Chen He,Zhou Minyue,Liu Yaoqiang...&Liu Xuqian.(2021).Identification and validation of potential novel biomarkers for oral squamous cell carcinoma..BIOENGINEERED,12,(1)
MLA：
Zhang Mengxue,et al."Identification and validation of potential novel biomarkers for oral squamous cell carcinoma.".BIOENGINEERED 12..1(2021):8845-8862
