机构：[1]Department of Stomatology, the Fourth Hospital of Hebei Medical University, No.12 Jiankang Road, Shijiazhuang, Hebei province 050000, China.临床科室口腔科河北医科大学第四医院[2]Department of Stomatology, the Third Hospital of Hebei Medical University, No.139 Ziqiang Road, Shijiazhuang, Hebei province 050000, China.[3]Department of Stomatology, Shijiazhuang Second Hospital, No.53 Huaxi Road, Shijiazhuang, Hebei province 050000, China.[4]Department of Stomatology, Wei County People's Hospital, No.361 Kaifangxi Road, Xingtai, Hebei province 054700, China.
Background: Oral squamous cell carcinoma (OSCC) is a common malignancy in the oral cavity that represents a significant global health problem. Multivariate analysis has shown that long non-coding RNA LINC01296 plays an important role in cancer biology. However, the functions of LINC01296 in OSCC are still unknown. Methods: The RNA profiles and clinicopathological information of OSCC patients and healthy subjects were downloaded. The expression level and prognostic value of LINC01296 were assessed. The functions and pathways of LINC01296were explored using the Gene Set Enrichment Analysis (GSEA) and functional analysis. The expression of LINC01296 in OSCC tissues and cell lines was determined by RT-qPCR. MTS assay was used to evaluate cell growth. The migration and invasion capacities of cells were assessed by wound healing assay and Transwell assay. Results: LINC01296 was overexpressed in the TCGA-OSCC datasets. High LINC01296expression was strongly correlated with poor outcomes of OSCC patients. LINC01296 was overexpressed in OSCC tissues compared with para-carcinoma tissues. Moreover, the expression of linc01296 was higher in CAL-27, HSC-2, and SCC-25 cells than in normal human oral keratinocytes (NHOKs). Functional analysis suggested that LINC01296might be involved in the regulation of the Wnt and MAPK signaling pathways. Additionally, LINC01296 deficiency suppressed the growth, migration, and invasion of OSCC cells, whereas overexpression of TFAP2A-AS1 cause opposite results. Conclusion: Our study showed that LINC01296 promoted OSCC cell growth, migration, and invasion, suggesting that LINC01296 might be a potential therapeutic target for OSCC.
第一作者机构：[1]Department of Stomatology, the Fourth Hospital of Hebei Medical University, No.12 Jiankang Road, Shijiazhuang, Hebei province 050000, China.
通讯作者：
推荐引用方式(GB/T 7714)：
Yang Kaicheng,Li Lei,Chen Yanping,et al.The role of long non-coding RNA LINC01296 in oral squamous cell carcinoma: a study based on bioinformatics analysis and in vitro validation[J].JOURNAL OF CANCER.2022,13(3):775-783.doi:10.7150/jca.60417.
APA：
Yang, Kaicheng,Li, Lei,Chen, Yanping,Man, Shasha,Yang, Lei...&Zhao, Jianguang.(2022).The role of long non-coding RNA LINC01296 in oral squamous cell carcinoma: a study based on bioinformatics analysis and in vitro validation.JOURNAL OF CANCER,13,(3)
MLA：
Yang, Kaicheng,et al."The role of long non-coding RNA LINC01296 in oral squamous cell carcinoma: a study based on bioinformatics analysis and in vitro validation".JOURNAL OF CANCER 13..3(2022):775-783
