The miR-141-3p has been reported to participate in regulating autophagy and tumor-stroma interactions in ovarian cancer (OC). We aim to investigate whether miR-141-3p accelerates the progression of OC and its effect on macrophage 2 polarization by targeting the Kelch-like ECH-associated protein1-Nuclear factor E2-related factor2 (Keap1-Nrf2) pathway. SKOV3 and A2780 cells were transfected with miR-141-3p inhibitor and negative control to confirm the regulation of miR-141-3p on OC development. Moreover, the growth of tumors in xenograft nude mice treated by cells transfected with miR-141-3p inhibitor was established to further testify the role of miR-141-3p in OC. The expression of miR-141-3p was higher in OC tissue compared with non-cancerous tissue. Downregulation of miR-141-3p inhibited the proliferation, migration, and invasion of ovarian cells. Furthermore, miR-141-3p inhibition also suppressed M2-like macrophage polarization and in vivo OC progression. Inhibition of miR-141-3p significantly enhanced the expression of Keap1, the target gene of miR-141-3p, and thus downregulated Nrf2, while activation of Nrf2 reversed the reduction in M2 polarization by miR-141-3p inhibitor. Collectively, miR-141-3p contributes to tumor progression, migration, and M2 polarization of OC by activating the Keap1-Nrf2 pathway. Inhibition of miR-141-3p attenuates the malignant biological behavior of ovarian cells by inactivating the Keap1-Nrf2 pathway.
基金:
project of"miRNA-29 expression on tumor-related macro-phage polarization in ovarian cancer" from Hebei Provincial Department of Health [20221320]
第一作者机构:[2]Hebei Med Univ, Hosp 4, Dept Reprod Med, Shijiazhuang 050011, Hebei, Peoples R China
通讯作者:
通讯机构:[1]Hebei Med Univ, Hosp 4, Dept Gynecol, 12 Jiankang Rd, Shijiazhuang 050011, Hebei, Peoples R China
推荐引用方式(GB/T 7714):
Zhao Jingyun,Liu Leilei,Zhao Wei,et al.miR-141-3p accelerates ovarian cancer progression and promotes M2-like macrophage polarization by targeting the Keap1-Nrf2 pathway[J].OPEN MEDICINE.2023,18(1):doi:10.1515/med-2023-0729.
APA:
Zhao, Jingyun,Liu, Leilei,Zhao, Wei,Lv, Cuiting,Zhang, Na...&Zhang, Zhengmao.(2023).miR-141-3p accelerates ovarian cancer progression and promotes M2-like macrophage polarization by targeting the Keap1-Nrf2 pathway.OPEN MEDICINE,18,(1)
MLA:
Zhao, Jingyun,et al."miR-141-3p accelerates ovarian cancer progression and promotes M2-like macrophage polarization by targeting the Keap1-Nrf2 pathway".OPEN MEDICINE 18..1(2023)