机构：[1]Department of Respiration, Hebei Provincial Hospital of Traditional Chinese Medicine, Shi Jiazhuang, China[2]Department of Clinical Laboratory, Qingdao Woman and Children’s Hospital, Qingdao, China[3]Tumour Institute, Fourth Hospital of Hebei Medical University, Shi Jiazhuang, China河北医科大学第四医院
Introduction: Recent studies have shown that long non-coding RNAs (lncRNAs) are involved in a variety of biological processes and diseases in humans, including cancer. However, the exact effects and molecular mechanisms of TP73-AS1 in non-small cell lung cancer (NSCLC) progression are still unknown. The present study is aimed to reveal the detailed functions and the mechanism of TP73-AS1 in the regulation of NSCLC cell proliferation. Material and methods: TP73-AS1 expression in NSCLC tissues and cell lines was determined using real-time PCR assays. The functions of TP73-AS1 in the regulation of NSCLC cell proliferation was evaluated using BrdU assays. The interaction between TP73-AS1 and miR-141-3p was confirmed using luciferase report gene assays. Results: TP73-AS1 was upregulated in NSCLC tissues and cell lines. However, when knockdown of TP73-AS1 inhibited the NSCLC proliferation. By using online tools, we screened out miR-141-3p may combined with TP73-AS1. With use of luciferase assays, we confirmed that miR-141-3p could directly bind to TP73-AS1. In NSCLC tissues, miR-141-3p was down-regulated; TP73-AS1 was inversely correlated with miR-141-3p. Conclusions: Our data suggest that TP73-AS1 might be an oncogenic lncRNA that promotes proliferation of NSCLC and might be regarded as a therapeutic target in NSCLC.
第一作者机构：[1]Department of Respiration, Hebei Provincial Hospital of Traditional Chinese Medicine, Shi Jiazhuang, China
通讯作者：
通讯机构：[3]Tumour Institute, Fourth Hospital of Hebei Medical University, Shi Jiazhuang, China[*1]Tumour Institute Fourth Hospital of Hebei Medical University 169 Tianshan Rd Shi Jiazhuang 050011, China
推荐引用方式(GB/T 7714)：
Liu Xinfa,Wang Mingming,Cui Yanzhi.LncRNA TP73-AS1 interacted with miR-141-3p to promote the proliferation of non-small cell lung cancer[J].ARCHIVES OF MEDICAL SCIENCE.2019,15(6):1547-1554.doi:10.5114/aoms.2019.86820.
APA：
Liu, Xinfa,Wang, Mingming&Cui, Yanzhi.(2019).LncRNA TP73-AS1 interacted with miR-141-3p to promote the proliferation of non-small cell lung cancer.ARCHIVES OF MEDICAL SCIENCE,15,(6)
MLA：
Liu, Xinfa,et al."LncRNA TP73-AS1 interacted with miR-141-3p to promote the proliferation of non-small cell lung cancer".ARCHIVES OF MEDICAL SCIENCE 15..6(2019):1547-1554
