机构：[1]Department of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011河北医科大学第四医院胸五科临床科室[2]Department of Thoracic Surgery, Affiliated Hospital of Hebei University, Baoding, Hebei 071000[3]Department of Thoracic Surgery, Xingtai People's Hospital, Xingtai, Hebei 054000, P.R. China
Emerging studies have indicated that microRNAs (miRNAs/miRs) are involved in regulating non-small cell lung cancer (NSCLC)-associated processes. The present study aimed to evaluate the biological roles of miR-374a-5p in NSCLC. Using reverse transcription-quantitative PCR, the expression levels of miR-374a-5p were determined in NSCLC cells and a normal cell line. Functional experiments were performed to investigate the functions of miR-374a-5p in NSCLC. A luciferase activity reporter assay and rescue experiments were performed to validate NCK adaptor protein 1 (NCK1) as a functional target of miR-374a-5p. It was demonstrated that miR-374a-5p levels were decreased in NSCLC cell lines compared with those in a normal cell line. Furthermore, overexpression of miR-374a-5p inhibited NSCLC cell proliferation and migration in vitro. Of note, NCK1 overexpression reversed the effects of miR-375a-5p on NSCLC cell proliferation and migration. The present results confirmed the tumor suppressor role of miR-374a-5p via targeting NCK1 in NSCLC, indicating the importance of the miR-374a-5p/NCK1 axis in NSCLC.
基金：
Pre-clinical Transformation Research of panel, a Multigene Molecular Marker for Early Diagnosis of Lung Adenocarcinoma ('Office-School Consultation Fund-Science and Technology Innovation' Project of Hebei Science and Technology Department-Hebei Medical Uni [2020TXZH04]
第一作者机构：[1]Department of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011[2]Department of Thoracic Surgery, Affiliated Hospital of Hebei University, Baoding, Hebei 071000
通讯作者：
通讯机构：[1]Department of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011[*1]Department of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, 12 Jiankang Road, Shijiazhuang, Hebei 050011, P.R. China
推荐引用方式(GB/T 7714)：
Guo Qiang,Wang Haijun,Xu Yanzhao,et al.miR-374a-5p inhibits non-small cell lung cancer cell proliferation and migration via targeting NCK1[J].EXPERIMENTAL AND THERAPEUTIC MEDICINE.2021,22(3):doi:10.3892/etm.2021.10375.
APA：
Guo, Qiang,Wang, Haijun,Xu, Yanzhao,Wang, Mingbo&Tian, Ziqiang.(2021).miR-374a-5p inhibits non-small cell lung cancer cell proliferation and migration via targeting NCK1.EXPERIMENTAL AND THERAPEUTIC MEDICINE,22,(3)
MLA：
Guo, Qiang,et al."miR-374a-5p inhibits non-small cell lung cancer cell proliferation and migration via targeting NCK1".EXPERIMENTAL AND THERAPEUTIC MEDICINE 22..3(2021)
