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Identification of the immunogenic membrane proteins, Catalase, PgbA, and PgbB, as potential antigens against Helicobacter pylori

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机构： [1]Research Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, China. [2]Provincial Center for Clinical Laboratories, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, China. [3]Shijiazhuang Center for Disease Control and Prevention, Shijiazhuang, Hebei 050000, China. [4]Clinical College, Hebei Medical University, Shijiazhuang, Hebei 050020, China. [5]Department of Gastroenterology, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, China.

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关键词： Helicobacter pylori vaccine candidate antibody membrane protein

摘要：

This study aims to investigate the specific membrane antigens that are targeted by antibodies raised against H. pylori.Bovine milk antibodies were prepared using whole H. pylori, purified membrane proteins, or both. Enzyme-linked immunosorbent assay (ELISA) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) experiments revealed that these immunogens triggered anti-H. pylori antibody production in milk. The highest antibody titer was induced by the mixture of whole bacteria and purified membrane proteins. The antibodies induced by mixed immunogens significantly inhibited H. pylori growth in vitro and were used to identify catalase, plasminogen-binding protein A (PgbA), and PgbB via western blotting, immunoprecipitation and two-dimensional western blotting (2D-WB) followed by LC-MS/MS. The immunogenicity of PgbA and PgbB was verified in mice vaccinated with their B-cell epitope vaccines. Following prophylactic vaccination of C57BL/6 mice, each of the three antigens alone and their combination reduced the weight loss in mice, increased antibody titers, and relieved the inflammatory status of the gastric mucosa following H. pylori infection.Catalase, PgbA, and PgbB could serve as valuable membrane antigens for the development of anti-H. pylori immunotherapies.© The Author(s) 2023. Published by Oxford University Press on behalf of Applied Microbiology International.

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出版当年[2023]版：

大类 | 3 区生物学

小类 | 3 区生物工程与应用微生物 3 区微生物学

最新[2025]版：

大类 | 3 区生物学

小类 | 3 区生物工程与应用微生物 4 区微生物学

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出版当年[2023]版：

Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Q2 MICROBIOLOGY

最新[2024]版：

Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Q2 MICROBIOLOGY

影响因子： 3.2 最新[2024版] 3.8 最新五年平均 3.2 出版当年[2023版] 4 出版当年五年平均 4 出版前一年[2022版] 3.2 出版后一年[2024版]

第一作者：

第一作者机构： [1]Research Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, China. [2]Provincial Center for Clinical Laboratories, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, China.

通讯作者：

通讯机构： [1]Research Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, China.

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Identification of the immunogenic membrane proteins, Catalase, PgbA, and PgbB, as potential antigens against Helicobacter pylori

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