机构：[1]Department of Nuclear Medicine, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China医技科室核医学科河北医科大学第四医院[2]Department of Neurology, The Second Hospital of Hebei Medical University, Shijiazhuang 050011, China
Purpose This study investigated a novel SPECT agent for the noninvasive imaging of EGFR-overexpressing tumors. Methods The EGFR-targeting peptide GE11 was synthesized with the introduction of four amino acids (GGGC) to its C-terminal to act as a strong chelator and radiolabeled using(99m)Tc. The radiochemical yield of the(99m)Tc-peptide-GE11 were evaluated using RP-HPLC. Cellular assays of the probe were performed on two NSCLC cell lines: A549 (high expression) and H23 (low expression). Biodistribution and SPECT imaging were performed in BALB/c nude mice bearing A549 and H23 NSCLC xenografts. Results The(99m)Tc-peptide-GE11 was prepared at high efficiency with radiochemical yield of 98.40 +/- 1.00 % and it showed favorable stability. The cellular uptake was significantly higher in A549 than in H23 at all time points (especially at 1 h, which was 10.34 +/- 0.72 and 2.04 +/- 0.18, respectively). A nearly 56% reduction in probe uptake was observed after pretreatment with excess unlabeled peptides. The performance of SPECT imaging and biodistribution demonstrated higher uptake of the(99m)Tc-peptide-GE11 in A549 xenograft than in H23 xenografts. Conclusion The new SPECT tracer(99mT)c-peptide-GE11 showed EGFR specificity, favorable pharmacokinetics and great potential for EGFR-targeted imaging.
基金：
This work was supported by the National Natural Science Foundation of China (NSFC)
under Grant (NO. 81571702).
语种：
外文
被引次数：
WOS：
PubmedID：
中科院分区：
出版当年[2020]版：
大类|4 区医学
小类|2 区核科学技术3 区生物学4 区核医学
最新[2025]版：
大类|4 区医学
小类|3 区核科学技术4 区生物学4 区核医学
JCR分区：
出版当年[2020]版：
Q1NUCLEAR SCIENCE & TECHNOLOGYQ2BIOLOGYQ3RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
最新[2024]版：
Q1NUCLEAR SCIENCE & TECHNOLOGYQ2BIOLOGYQ2RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
第一作者机构：[1]Department of Nuclear Medicine, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China[2]Department of Neurology, The Second Hospital of Hebei Medical University, Shijiazhuang 050011, China
通讯作者：
通讯机构：[1]Department of Nuclear Medicine, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China[*1]Department of Nuclear Medicine, The Fourth Hospital of Hebei Medical University, JianKang Road, Shijiazhuang, 050011, P. R. China.
推荐引用方式(GB/T 7714)：
Jiao Honglei,Zhao Xinming,Han Jingya,et al.Synthesis of a novel(99m)Tc labeled GE11 peptide for EGFR SPECT imaging[J].INTERNATIONAL JOURNAL OF RADIATION BIOLOGY.2020,96(11):1443-1451.doi:10.1080/09553002.2020.1811419.
APA：
Jiao, Honglei,Zhao, Xinming,Han, Jingya,Zhang, Jingmian&Wang, Jianfang.(2020).Synthesis of a novel(99m)Tc labeled GE11 peptide for EGFR SPECT imaging.INTERNATIONAL JOURNAL OF RADIATION BIOLOGY,96,(11)
MLA：
Jiao, Honglei,et al."Synthesis of a novel(99m)Tc labeled GE11 peptide for EGFR SPECT imaging".INTERNATIONAL JOURNAL OF RADIATION BIOLOGY 96..11(2020):1443-1451
