The clinical significance of methylation of MAGE-A1 and-A3 promoters and expression of DNA methyltransferase in patients with laryngeal squamous cell carcinoma
机构：[1]Hebei Med Univ, Hosp 4, Dept Otolaryngol, Shijiazhuang, Hebei, Peoples R China临床科室耳鼻喉科河北医科大学第四医院[2]Hebei Med Univ, Hosp 4, Dept Res Ctr, Shijiazhuang, Hebei, Peoples R China河北医科大学第四医院[3]Hebei Med Univ, Hosp 4, Tumor Res Inst, Shijiazhuang, Hebei, Peoples R China河北医科大学第四医院
Purpose: Abnormal DNA methylation plays an important role in clinical diagnosis and prognosis of various tumors. DNA methylation is catalyzed by DNA methyltransferase (DNMT). However, the methylation status of MAGE-A1 and MAGE-A3 promoter regions in LSCC is unclear. To investigate the methylation levels of MAGE-A1, -A3 in LSCC and corresponding normal tissues. The expression of DNMTs (DNMT1, DNMT3a and DNMT3b) in LSCC and the relationship between the methylation status of MAGE-A1, -A3 were analyzed. Materials and methods: We examined methylation status of MAGE-A1, -A3 in LSCC by using MSP. Meanwhile, the expression level of DNMTs in LSCC was detected by immunohistochemistry. And further analysis the correlation between DNMTs expression level and methylation status of MAGE-A1 and MAGE-A3. Results: The unmethylation rate of MAGE-A1, -A3 were 39.62% and 46.23%. The expression of DNMTs was 33.02% to 37.74%. The level of demethylation of MAGE-A1 and MAGE-A3 were negative related to DNMTs protein. MAGE-A1 and MAGE-A3 unmethylation status and DNMT3a expression were independent prognostic factors for LSCC. Conclusions: The DNMTs may participate in the methylation process of MAGE-A1 and MAGE-A3, which may play an important role in the occurrence and development of LSCC.
基金：
Health Department of Hebei Province [20150786]; Financial Department of Hebei Province [361006]
第一作者机构：[1]Hebei Med Univ, Hosp 4, Dept Otolaryngol, Shijiazhuang, Hebei, Peoples R China[2]Hebei Med Univ, Hosp 4, Dept Res Ctr, Shijiazhuang, Hebei, Peoples R China
通讯作者：
通讯机构：[2]Hebei Med Univ, Hosp 4, Dept Res Ctr, Shijiazhuang, Hebei, Peoples R China[3]Hebei Med Univ, Hosp 4, Tumor Res Inst, Shijiazhuang, Hebei, Peoples R China[*1]Research Center, the Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050017, People's Republic of China
推荐引用方式(GB/T 7714)：
Liu Shenghui,Zhao Yan,Xu Yuru,et al.The clinical significance of methylation of MAGE-A1 and-A3 promoters and expression of DNA methyltransferase in patients with laryngeal squamous cell carcinoma[J].AMERICAN JOURNAL OF OTOLARYNGOLOGY.2020,41(1):doi:10.1016/j.amjoto.2019.102318.
APA：
Liu, Shenghui,Zhao, Yan,Xu, Yuru,Sang, Meixiang,Zhao, Ruili...&Shan, Baoen.(2020).The clinical significance of methylation of MAGE-A1 and-A3 promoters and expression of DNA methyltransferase in patients with laryngeal squamous cell carcinoma.AMERICAN JOURNAL OF OTOLARYNGOLOGY,41,(1)
MLA：
Liu, Shenghui,et al."The clinical significance of methylation of MAGE-A1 and-A3 promoters and expression of DNA methyltransferase in patients with laryngeal squamous cell carcinoma".AMERICAN JOURNAL OF OTOLARYNGOLOGY 41..1(2020)
