机构:[1]GuangDong Women & Children Hosp, Dept Ophtalmol, Guangzhou, Guangdong, Peoples R China[2]GuangZhou Medcial Univ, Affiliated Hosp 1, Dept Anesthesiol, Guangzhou, Guangdong, Peoples R China[3]GuangDong Women & Children Hosp, Dept Lab, Guangzhou, Guangdong, Peoples R China[4]Hebei Med Univ, Hosp 4, Dept Ophthalmol, Shijiazhuang, Hebei, Peoples R China临床科室眼科河北医科大学第四医院[5]Guangdong Med Univ, Affiliated Hosp, Dept Oncol, Zhanjiang, Peoples R China
OBJECTIVE: In this study, we aimed at investigating whether MEG3 may be involved in the pathogenesis of glaucoma by regulating the autophagy of retinal ganglion cells (RGCs). MATERIALS AND METHODS: We used qRTPCR to detect the expression of MEG3 in RGC-5s cell line under high hydrostatic pressure. RGC-5s were transfected with a lentiviral vector to achieve MEGS overexpression or knockdown. The influence of overexpression or inhibition of MEG3 on cell proliferation and apoptosis was observed using CCK-8 test and flow cytometry. After overexpression of MEG3 and/or knockdown of MEG3 or Beclin-1, detection of the expressions of autophagy-related and apoptosis-related proteins was performed using Western blot. RESULTS: MEG3 expression level increased in RGC-5 cells under high hydrostatic pressure, while exogenously decreased MEG3 expression can reverse the impact of the high pressure on RGC-5 cells. Additionally, overexpression of MEG3 can improve Atg3 expression, promote cell apoptosis, inhibit cell proliferation, and enhance autophagy levels. Meanwhile, knockdown of Beclin-1 up-regulated Bcl-2 level. CONCLUSIONS: Upregulation of MEG3 is involved in the pathogenesis of glaucoma through promoting apoptosis of retinal ganglion cells, the mechanism of which may be related to the enhanced autophagy levels.
第一作者机构:[1]GuangDong Women & Children Hosp, Dept Ophtalmol, Guangzhou, Guangdong, Peoples R China
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推荐引用方式(GB/T 7714):
Sun W.,Li Y-N.,Ye J-F.,et al.MEG3 is involved in the development of glaucoma through promoting the autophagy of retinal ganglion cells[J].EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES.2018,22(9):2534-2540.doi:10.26355/eurrev_201805_14942.
APA:
Sun, W.,Li, Y-N.,Ye, J-F.,Guan, Y-Q.&Li, S-J..(2018).MEG3 is involved in the development of glaucoma through promoting the autophagy of retinal ganglion cells.EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES,22,(9)
MLA:
Sun, W.,et al."MEG3 is involved in the development of glaucoma through promoting the autophagy of retinal ganglion cells".EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES 22..9(2018):2534-2540