Trastuzumab (TZM) significantly improves the outcomes of patients with breast cancer; however, it is associated with severe cardiotoxicity. Ginsenoside Rg2 was reported to exert protective effects against myocardial injury and apoptosis in human cardiomyocytes (HCMs). However, whether ginsenoside Rg2 protects HCMs against TZM-induced toxicity remains unclear. The present study investigated the proliferation of HCMs using a Cell Counting Kit-8 assay and Ki67 immunofluorescence staining. Apoptotic cells were detected by Annexin V/propidium iodide staining and flow cytometry. Furthermore, monodansylcadaverine staining was performed to detect cell autophagy. In addition, western blotting was used to detect the expression levels of phosphorylated (p)-Akt, p-mTOR, beclin 1, microtubule associated protein 1 light chain 3 alpha (LC3) and autophagy protein 5 (ATG5) in HCMs. Pretreatment with ginsenoside Rg2 significantly protected HCMs against TZM-induced cytotoxicity by inhibiting apoptosis. Furthermore, pretreatment with ginsenoside Rg2 induced autophagy in HCMs by upregulating the expression levels of p-Akt, p-mTOR, beclin 1, LC3 and ATG5. The results obtained in the present study suggested that ginsenoside Rg2 could protect HCMs against TZM-induced cardiotoxicity by activating autophagy. Therefore, ginsenoside Rg2 may serve as a potential therapeutic agent to prevent TZM-related cardiotoxicity in patients with breast cancer.
第一作者机构:[1]Hebei Med Univ, Dept Cardiovasc Med, Shijiazhuang, Hebei, Peoples R China
通讯作者:
通讯机构:[2]Hebei Gen Hosp, Dept Cardiovasc Med, 348 West Heping Rd, Shijiazhuang 050000, Hebei, Peoples R China[*1]Department of Cardiovascular Medicine, Hebei General Hospital, 348 West Heping Road, Shijiazhuang, Hebei 050000, P.R. China
推荐引用方式(GB/T 7714):
Liu Guang,Qi Xiaoyong,Li Xingtao,et al.Ginsenoside Rg2 protects cardiomyocytes against trastuzumab-induced toxicity by inducing autophagy[J].EXPERIMENTAL AND THERAPEUTIC MEDICINE.2021,21(5):doi:10.3892/etm.2021.9904.
APA:
Liu, Guang,Qi, Xiaoyong,Li, Xingtao&Sun, Fangyi.(2021).Ginsenoside Rg2 protects cardiomyocytes against trastuzumab-induced toxicity by inducing autophagy.EXPERIMENTAL AND THERAPEUTIC MEDICINE,21,(5)
MLA:
Liu, Guang,et al."Ginsenoside Rg2 protects cardiomyocytes against trastuzumab-induced toxicity by inducing autophagy".EXPERIMENTAL AND THERAPEUTIC MEDICINE 21..5(2021)