机构：[1]Department of Endoscopy,The Fourth Hospital of Hebei Medical University, Shijiazhuang, China医技科室内镜科河北医科大学第四医院[2]The Third Department of Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China临床科室外三科河北医科大学第四医院[3]Department of Biochemistry and Molecular Biology, Hebei Medical University, Shijiazhuang, China
This study aimed to investigate the role of insulin-like growth factor II mRNA binding protein 3 (IMP3) in neuroendocrine tumor (NET). Mouse NET STC-1 cell line was chosen as the experimental model and three IMP3-targeting siRNAs and a non-specific scramble siRNA were transfected into STC-1 cells. The efficiency of IMP3 siRNA to knockdown IMP3 was evaluated by immunocytochemical staining. Cell proliferation was detected by MTT assay. Cell migration and invasion was analyzed with Transwell chamber assay. Protein expression was detected by Western blot analysis. We found that IMP3 silencing inhibited the proliferation of STC-1 cells potentially by down-regulating the expression of cell proliferation associated proteins EGFR and Ki67. Furthermore, IMP3 silencing inhibited the migration and invasion of STC-1 cells potentially by downregulating the expression of metastasis associated proteins IGF1R, MMP2 and MMP9. In conclusion, this study provides the first evidence that IMP3 plays an oncogenic role in Net and is a promising therapeutic target for NET.
基金：
National Natural
Science Foundation of China (No. 81072033
and 81372580); Natural Science Foundation of Hebei Province (No. C2010000619); and
Key Medical Research Program of Hebei (No.
20160620).
第一作者机构：[1]Department of Endoscopy,The Fourth Hospital of Hebei Medical University, Shijiazhuang, China
通讯作者：
通讯机构：[2]The Third Department of Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China[*1]Department of General Surgery, The Fourth Hospital of Hebei Medical University, 12 Jiankang Road, Shijiazhuang 050011, Hebei Province, China.
推荐引用方式(GB/T 7714)：
Er Li-Mian,Li Yong,Wu Ming-Li,et al.Insulin-like growth factor II mRNA binding protein 3 regulates proliferation, invasion and migration of neuroendocrine cancer cells[J].INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY.2017,10(10):10269-10275.
APA：
Er, Li-Mian,Li, Yong,Wu, Ming-Li,Li, Bin,Tan, Bi-Bo...&Wang, Shi-Jie.(2017).Insulin-like growth factor II mRNA binding protein 3 regulates proliferation, invasion and migration of neuroendocrine cancer cells.INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,10,(10)
MLA：
Er, Li-Mian,et al."Insulin-like growth factor II mRNA binding protein 3 regulates proliferation, invasion and migration of neuroendocrine cancer cells".INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY 10..10(2017):10269-10275
