机构：[1]Hebei Univ Chinese Med, Dept Pharmacol, 3 Xingyuan Rd, Shijiazhuang 050200, Hebei, Peoples R China[2]Hebei Med Univ, Hosp 4, Dept Pharm, Shijiazhuang, Peoples R China药学部药学部河北医科大学第四医院[3]Maternal & Child Hlth Hosp, Dept Pharm, Tangshan, Peoples R China[4]Hebei Med Univ, Hosp 2, East Branch, Vasc Surg, Shijiazhuang, Hebei, Peoples R China[5]Air Force Gen Hosp, Intens Care Unit, Beijing, Peoples R China[6]China Pharmaceut Univ, Key Lab Drug Metab & Pharmacokinet, Nanjing, Jiangsu, Peoples R China
ObjectivesThis study investigated the effect of tannic acid (TA), a plant-derived hydrolyzable polyphenol, on Kv7.4 and Kv7.5 K+ channels and rat mesenteric artery. MethodsWhole-cell patch clamp experiments were used to record the Kv7.4 and Kv7.3/7.5 K+ currents expressed in HEK293 cells; and the tension changes of mesenteric arteries isolated from rats were recorded using small vessel myography apparatus. Key findingsTannic acid increases the Kv7.4 and Kv7.3/7.5 K+ currents in a concentration-dependent manner (median effective concentration (EC50) = 27.3 3.6 m and EC50 = 23.1 +/- 3.9 m, respectively). In addition, 30 m TA shifts the G-V curve of Kv7.4 and Kv7.3/7.5 K+ currents to the left by 14.18 and 25.24 mV, respectively, and prolongs the deactivation time constants by 184.44 and 154.77 ms, respectively. Moreover, TA relaxes the vascular tension of rat mesenteric arteries in a concentration-dependent manner (half inhibitory concentration (IC50) = 148.7 +/- 13.4 m). ConclusionThese results confirms the vasodilatory effects of TA on rat mesenteric artery and the activating effects on the Kv7.4 and Kv7.3/7.5 K+ channels, which may be a mechanism to explain the vasodilatory effect and this mechanism can be used in the research of antihypertension.
基金：
Research Foundation of the Education Bureau of Heibei Province [grant number
QN20131046]; the National Natural Science Foundation of
China [grant number 31401003] and the Youth Foundation
of Hebei University of Chinese Medicine [grant number
QNZ2015003].
第一作者机构：[1]Hebei Univ Chinese Med, Dept Pharmacol, 3 Xingyuan Rd, Shijiazhuang 050200, Hebei, Peoples R China
通讯作者：
通讯机构：[1]Hebei Univ Chinese Med, Dept Pharmacol, 3 Xingyuan Rd, Shijiazhuang 050200, Hebei, Peoples R China[*1]Department of Pharmacology, Hebei University of Chinese Medicine, No. 3, Xingyuan Road, Shijiazhuang 050200, Hebei, China
推荐引用方式(GB/T 7714)：
Zhang Yuanyuan,Chu Xi,Liu Ling,et al.Tannic acid activates the Kv7.4 and Kv7.3/7.5 K+ channels expressed in HEK293 cells and reduces tension in the rat mesenteric arteries[J].JOURNAL OF PHARMACY AND PHARMACOLOGY.2016,68(4):494-502.doi:10.1111/jphp.12527.
APA：
Zhang, Yuanyuan,Chu, Xi,Liu, Ling,Zhang, Nan,Guo, Hui...&Zhang, Jianping.(2016).Tannic acid activates the Kv7.4 and Kv7.3/7.5 K+ channels expressed in HEK293 cells and reduces tension in the rat mesenteric arteries.JOURNAL OF PHARMACY AND PHARMACOLOGY,68,(4)
MLA：
Zhang, Yuanyuan,et al."Tannic acid activates the Kv7.4 and Kv7.3/7.5 K+ channels expressed in HEK293 cells and reduces tension in the rat mesenteric arteries".JOURNAL OF PHARMACY AND PHARMACOLOGY 68..4(2016):494-502
