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A key mediator, PTX3, of IKK/I kappa B/NF-kappa B exacerbates human umbilical vein endothelial cell injury and dysfunction

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收录情况： ◇ SCIE

作者：

机构： [1]Department of Cardiovascular Surgery, Fourth Hospital of Hebei Medical University, Shijiazhuang, China [2]Department of Neurology, Hebei General Hospital, Shijiazhuang, China

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关键词： PTX3 NF-kappa B iNOS atherosclerosis HUVECs

摘要：

Objective: This study was performed to investigate PTX3-mediated iNOS expression and IKK/I kappa B/NF-kappa B activation in PA-induced atherosclerotic HUVECs injury model. Methods: The cell viability was detected by the CCK8 assay. The cell apoptosis was assessed by annexin V-PI double-labeling staining. Expression of genes and proteins were analyzed by real-time PCR and western blotting respectively. Cells were transfected with siRNAs as a gene silencing methods. Results: PA induced cell apoptosis in human umbilical vein endothelial cells in a time and dose-dependent manner. PA also induced upregulation expression of PTX3. TPCA-1, an inhibitor of IKK-2, could suppress the expression of PTX3 and phospho-I kappa B-alpha in PA-induced endothelial dysfunction cell model. We also found that transfection of cells with PTX3 siRNA reduced the expression of iNOS and NO, and protected PA-induced cell apoptosis in HUVECs. Conclusions: PTX3 could exacerbate endothelial dysfunction, at least partially, through IKK/I kappa B/NF-kappa B activation and overexpression of iNOS and NO, and advance the development of atherosclerosis.

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出版当年[2014]版：

大类 | 4 区医学

小类 | 4 区肿瘤学 4 区病理学

最新[2025]版：

大类 | 4 区医学

小类 | 4 区肿瘤学 4 区病理学

JCR分区：

出版当年[2014]版：

Q3 PATHOLOGY Q3 ONCOLOGY

最新[2023]版：

Q3 PATHOLOGY Q4 ONCOLOGY

影响因子： 1.2 最新[2023版] 1.3 最新五年平均 1.891 出版当年[2014版] 2.001 出版当年五年平均 1.783 出版前一年[2013版] 1.581 出版后一年[2015版]

第一作者：

第一作者机构： [1]Department of Cardiovascular Surgery, Fourth Hospital of Hebei Medical University, Shijiazhuang, China

通讯作者：

通讯机构： [1]Department of Cardiovascular Surgery, Fourth Hospital of Hebei Medical University, Shijiazhuang, China [*1]Department of Cardiovascular Surgery, The Fourth Hospital of Hebei Medical University, Jiankang Road 12, Shijiazhuang 050011, Hebei, P. R. China

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