Background and Aim: Dopamine (DA) is considered to be an important modulator of enteric function. Recent experiments have suggested that DA receptors are widely expressed in animal gastrointestinal tract. The aim of this study was to explore the expression of DA receptors (D1R, D2R, D3R, D4R, D5R) in sling fibers and clasp fibers from the human lower esophageal sphincter (LES). Methods: Muscle strips of sling and clasp fibers from the LES were obtained from patients undergoing esophago-gastrectomy, and circular muscle strips from the esophagus and stomach were used as controls. Reverse transcription-polymerase chain reaction and western blotting were used to determine the expression of the five subtypes of DA receptors. Results: Messenger RNA and protein for three of five DA receptors were identified in the sling and clasp fibers of the LES. Expression was highest for D1R, then D5R and D2R in decreasing levels. D3R and D4R mRNAand protein were not identified in the muscle strips. Conclusion: D1R, D2R, D5R can be detected in the human LES, and probably contribute to LES function. D3R and D4R are not expressed, and probably do not contribute to LES function in humans.
基金：
China National
Natural Science Foundation, and a research
grant from the Education Department of
Hebei Province, China.
第一作者机构：[1]Department of Thoracic Surgery, Fourth Hospital, Hebei Medical University, Shijiazhuang, China
通讯作者：
通讯机构：[1]Department of Thoracic Surgery, Fourth Hospital, Hebei Medical University, Shijiazhuang, China[*1]Department of Thoracic Surgery, Fourth Hospital, Hebei Medical University, 12 Jiankang Road, Shijiazhuang, Hebei province 050011, China.
推荐引用方式(GB/T 7714)：
Liu Xin-Bo,Liu Jun-Feng.Expression of dopamine receptors in human lower esophageal sphincter[J].JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY.2012,27(5):945-950.doi:10.1111/j.1440-1746.2012.07100.x.
APA：
Liu, Xin-Bo&Liu, Jun-Feng.(2012).Expression of dopamine receptors in human lower esophageal sphincter.JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY,27,(5)
MLA：
Liu, Xin-Bo,et al."Expression of dopamine receptors in human lower esophageal sphincter".JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY 27..5(2012):945-950
