机构：[1]State Key Laboratory of Silkworm Genome Biology, Southwest University , Chongqing, China .[2]Department of Neurosurgery, Daping Hospital, Third Military Medical University , PLA, Chongqing, China .[3]Department of Radiology, Fourth Hospital of Hebei Medical University , Shijiazhuang, Hebei Province, China.医技科室放射科河北医科大学第四医院
The Sonic Hedgehog (Shh) signaling pathway is commonly activated in gastrointestinal cancer. However, our understanding of the Shh pathway in gastric cancer remains limited. Here we examined the effects of cyclopamine, a specific inhibitor of the Shh signaling pathway, on cell growth and proliferation in gastric primary cancer cells GAM-016 and the MKN-45 cell line. The results showed that the Shh signaling molecules SHH, PTCH, SMO, GLI1, and GLI2 were intact and activated in both types of cells. Furthermore, we observed that cyclopamine inhibited gastric cancer cell proliferation through cell cycle arrest and apoptosis. An in vivo study using NOD/SCID mouse xenografts demonstrated that cyclopamine significantly prevented tumor growth and development. Our study indicated that Shh signaling pathway could promote gastric cancer cell proliferation and tumor development, and blocking this pathway may be a potential strategy in gastric cancer treatment.
基金：
We appreciate Qinrui Wang from the Australian National
University for her kind editing of this manuscript. This study
was supported by the National Basic Research Program of
China (No. 2012cb114603), the Research Fund for the Doctoral
Program of High Education of China (20130182110003), and
the Fundamental Research Funds for the Central Universities
(XDJK2013B020). Author contributions: J.W. performed
most of the experiments; J.Z. was in charge of drafting the manuscript;
H.Z. participated in theMTT assay; Z.W. and H.G. participated
in the primary cell culture; Y.W. was in charge of the
primary tumor specimen; Q.X. took part in the experimental design;
and H.C. was responsible for the whole project. All authors
read and approved the final manuscript.
语种：
外文
PubmedID：
第一作者：
第一作者机构：[1]State Key Laboratory of Silkworm Genome Biology, Southwest University , Chongqing, China .
共同第一作者：
通讯作者：
通讯机构：[1]State Key Laboratory of Silkworm Genome Biology, Southwest University , Chongqing, China .[3]Department of Radiology, Fourth Hospital of Hebei Medical University , Shijiazhuang, Hebei Province, China.[*1]State Key Laboratory of Silkworm Genome Biology Southwest University Chongqing 400716 China[*2]Department of Radiology Fourth Hospital of Hebei Medical University Shijiazhuang, Hebei Province 050011 China
推荐引用方式(GB/T 7714)：
Wan Jianhua,Zhou Ji,Zhao Hailong,et al.Sonic hedgehog pathway contributes to gastric cancer cell growth and proliferation.[J].BioResearch open access.2014,3(2):53-9.doi:10.1089/biores.2014.0001.
APA：
Wan Jianhua,Zhou Ji,Zhao Hailong,Wang Mei,Wei Zhuanqin...&Cui Hongjuan.(2014).Sonic hedgehog pathway contributes to gastric cancer cell growth and proliferation..BioResearch open access,3,(2)
MLA：
Wan Jianhua,et al."Sonic hedgehog pathway contributes to gastric cancer cell growth and proliferation.".BioResearch open access 3..2(2014):53-9
