机构：[1]Hebei Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology,College of Life Sciences, Hebei Normal University, Shijiazhuang 050024,Hebei, People’s Republic of China[2]The Fourth Hospital of Hebei MedicalUniversity, Shijiazhuang 050011, Hebei, People’s Republic of China[3]Departmentof Pathogenic Biology, College of Basic Medicine, Hebei Medical University,Shijiazhuang 050017, Hebei, People’s Republic of China[4]InstrumentalAnalysis Center, Hebei Normal University, Shijiazhuang 050024, Hebei, People’sRepublic of China
Babesia is a protozoan parasite that infects red blood cells in some vertebrates. Some species of Babesia can induce zoonoses and cause considerable harm. As the largest immune organ in mammals, the spleen plays an important role in defending against Babesia infection. When infected with Babesia, the spleen is seriously injured but still actively initiates immunomodulatory responses.
To explore the molecular mechanisms underlying the immune regulation and self-repair of the spleen in response to infection, this study used data-independent acquisition (DIA) quantitative proteomics to analyse changes in expression levels of global proteins and in phosphorylation modification in spleen tissue after Babesia microti infection in mice.
After mice were infected with B. microti, their spleens were seriously damaged. Using bioinformatics methods to analyse dynamic changes in a large number of proteins, we found that the spleen still initiated immune responses to combat the infection, with immune-related proteins playing an important role, including cathepsin D (CTSD), interferon-induced protein 44 (IFI44), interleukin-2 enhancer-binding factor 2 (ILF2), interleukin enhancer-binding factor 3 (ILF3) and signal transducer and activator of transcription 5A (STAT5A). In addition, some proteins related to iron metabolism were also involved in the repair of the spleen after B. microti infection, including serotransferrin, lactoferrin, transferrin receptor protein 1 (TfR1) and glutamate-cysteine ligase (GCL). At the same time, the expression and phosphorylation of proteins related to the growth and development of the spleen also changed, including protein kinase C-δ (PKC-δ), mitogen-activated protein kinase (MAPK) 3/1, growth factor receptor-bound protein 2 (Grb2) and P21-activated kinase 2 (PAK2).
Immune-related proteins, iron metabolism-related proteins and growth and development-related proteins play an important role in the regulation of spleen injury and maintenance of homeostasis. This study provides an important basis for the diagnosis and treatment of babesiosis.
基金：
Natural Science Fund for Distinguished Young
Scholars of Hebei Normal University (No. L2017J04).
第一作者机构：[1]Hebei Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology,College of Life Sciences, Hebei Normal University, Shijiazhuang 050024,Hebei, People’s Republic of China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Xue Xiaomin,Ren Shuguang,Yang Xiaohong,et al.Protein regulation strategies of the mouse spleen in response to Babesia microti infection.[J].PARASITES & VECTORS.2021,14(1):doi:10.1186/s13071-020-04574-5.
APA：
Xue Xiaomin,Ren Shuguang,Yang Xiaohong,Masoudi Abolfazl,Hu Yuhong...&Liu Jingze.(2021).Protein regulation strategies of the mouse spleen in response to Babesia microti infection..PARASITES & VECTORS,14,(1)
MLA：
Xue Xiaomin,et al."Protein regulation strategies of the mouse spleen in response to Babesia microti infection.".PARASITES & VECTORS 14..1(2021)
