机构:[1]Department of Thoracic surgery, The First Affiliated Hospital of Xingtai Medical College, Xingtai, China[2]Department of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China临床科室胸心外科(胸外科 心脏血管外科)河北医科大学第四医院
Background: Long noncoding RNAs (lncRNAs) are emerging as key players in the development and progression of cancer. Several malignancies involve dysregulated long noncoding ribonucleic acids (lncRNAs) in non-small cell lung cancer cell growth and their aggressive phenotypes. LncRNA B4GALT1-AS1 is important in the advancement of various malignancies, although its contribution to non-small cell lung cancer (NSCLC) remains unexplored. Methods: LncRNA B4GALT1-AS1 in NSCLC tissues was detected and further validated in a cohort of non-small cell lung cancer tissues. The effects of lncRNA B4GALT1-AS1 on proliferation were determined by in vitro experiments. The B4GALT1-AS1-miR-144-3p-ZEB1 axis was assessed by dual-luciferase reporter and RNA immunoprecipitation (RIP) assays. Furthermore, the mechanism of B4GALT1-AS1 was investigated using loss-of-function assays in vitro. Results: We showed significant upregulation of B4GALT1-AS1 in cell lines and tissues of NSCLC. B4GALT1-AS1 knockdown impeded the in vitro proliferation-related characteristics of the NSCLC cells. The demonstration of the binding capacity of B4GALT1-AS1 and miR-144-3p was predicted by bioinformatics and luciferase reporter activity assay. The B4GALT1-AS1 and miR-144-3p interaction was shown by using rescue experiments. NSCLC has a positive association with its target, zinc finger e-box binding homeobox 1 (ZEB1). Conclusions: In summary, the progression of NSCLC was facilitated by lncRNA B4GALT1-AS1 via interaction with miR-144-3p and positive regulation of ZEB1 expression.
第一作者机构:[1]Department of Thoracic surgery, The First Affiliated Hospital of Xingtai Medical College, Xingtai, China
通讯作者:
通讯机构:[*1]Department of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, China
推荐引用方式(GB/T 7714):
Liu Shi-Wei,Yang Pu,Li Fan-Nian,et al.LncRNA B4GALT1-AS1 promotes non-small cell lung cancer cell growth via increasing ZEB1 level by sponging miR-144-3p[J].TRANSLATIONAL CANCER RESEARCH.2022,11(3):538-547.doi:10.21037/tcr-22-296.
APA:
Liu, Shi-Wei,Yang, Pu,Li, Fan-Nian,Dou, Rui-Gang,Liu, Jun-Xiao&Liu, Guang-Jie.(2022).LncRNA B4GALT1-AS1 promotes non-small cell lung cancer cell growth via increasing ZEB1 level by sponging miR-144-3p.TRANSLATIONAL CANCER RESEARCH,11,(3)
MLA:
Liu, Shi-Wei,et al."LncRNA B4GALT1-AS1 promotes non-small cell lung cancer cell growth via increasing ZEB1 level by sponging miR-144-3p".TRANSLATIONAL CANCER RESEARCH 11..3(2022):538-547