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Circ_0005231 promotes the progression of esophageal squamous cell carcinoma via sponging miR-383-5p and regulating KIAA0101

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机构: [1]Department of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, China [2]Department of Thoracic Surgery, Hebei Province Cangzhou Hospital of Integrated Traditional and Western Medicine, Hebei, China
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关键词: esophageal squamous cell carcinoma circ_0005231 miR-383-5p KIAA0101

摘要:
Background Circular RNAs (circRNAs) can act as key regulators in human cancers, including esophageal squamous cell carcinoma (ESCC). However, the role and mechanism of circ_0005231 in ESCC have not previously been reported. Methods RNA levels and protein levels were detected by real-time quantitative polymerase chain reaction (RT-qPCR) and Western blot assay, respectively. Cell proliferation was assessed by colony formation assay and 5-ethynyl-2'-deoxyuridine (EdU) assay. Wound healing and transwell assays were used to assess cell migration and invasion, respectively. The intermolecular interaction was predicted by bioinformatic analysis and verified by RNA immunoprecipitation (RIP), RNA pulldown and dual-luciferase reporter assays. Xenograft tumor model was used for exploring the biological function of circ_0005231 in vivo. Results Circ_0005231 was upregulated in ESCC plasma, tissues and cells. Cell proliferation, migration and invasion were significantly restrained by knockdown of circ_0005231 in ESCC cells. Circ_0005231 acted as a sponge of miR-383-5p, and circ_0005231 regulated ESCC cellular behavior by sponging miR-383-5p. Moreover, miR-383-5p directly targeted KIAA0101, and circ_0005231 positively regulated KIAA0101 expression by sponging miR-383-5p. Furthermore, circ_0005231 knockdown suppressed the malignant behavior of ESCC cells by downregulating KIAA0101. Importantly, knockdown of circ_0005231 blocked xenograft tumor growth in vivo. Conclusion Circ_0005231 acted as a sponge of miR-383-5p to promote ESCC progression by upregulating KIAA0101, which provided a potential therapeutic strategy for ESCC treatment.

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出版当年[2022]版:
大类 | 3 区 医学
小类 | 3 区 肿瘤学 3 区 呼吸系统
最新[2025]版:
大类 | 4 区 医学
小类 | 4 区 肿瘤学 4 区 呼吸系统
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出版当年[2022]版:
Q3 ONCOLOGY Q3 RESPIRATORY SYSTEM
最新[2024]版:
Q2 RESPIRATORY SYSTEM Q3 ONCOLOGY

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第一作者:
第一作者机构: [1]Department of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, China [2]Department of Thoracic Surgery, Hebei Province Cangzhou Hospital of Integrated Traditional and Western Medicine, Hebei, China
通讯作者:
通讯机构: [1]Department of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei, China [*1]Department of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, No. 12, Jiankang Road, Shijiazhuang City, Hebei Province, China.
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