机构：[1]Discipline of Surgery,School of Medicine,Faculty of Health Sciences,The University of Adelaide,Royal Adelaide Hospital,South Australia 5005,Australia[2]Department of Thoracic Surgery,Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,Hebei Province,China河北医科大学第四医院[3]Division of Tissue Pathology,Institute of Medical and Veterinary Science,Adelaide,South Australia 5000,Australia[4]School of Nursing and Midwifery,Flinders University,Bedford Park,South Australia 5042,Australia
AIM: To measure the frequency of DNA methylation of the tissue inhibitor of metalloproteinase 3 (TIMP3)promoter and relate this to any change of gene expression in esophageal squamous cell carcinoma in patients from a region of high incidence in China.METHODS: Cancer cell lines were treated with or without the demethylating reagent 5-aza-2'-deoxycytidine.Methylation of the TIMP3 promoter was assessed in three regions by melt curve analysis and its expression was assessed by real-time RT-PCR. Tumors and proximal resection margins were obtained from 64 patients with esophageal squamous cell carcinoma from a region of high incidence in China. Methylation was assessed by melt curve analysis and expression by immunohistochemistry.RESULTS: Methylation in one of the three promoter regions assessed correlated with gene silencing in esophageal cell lines. A degree of methylation of TIMP3 was found in only four esophageal squamous cell carcinomas, and partial loss of TIMP3 protein expression in just one.
基金：
The National Health and Medical Research Council of Australia
第一作者机构：[1]Discipline of Surgery,School of Medicine,Faculty of Health Sciences,The University of Adelaide,Royal Adelaide Hospital,South Australia 5005,Australia[*1]Discipline of Surgery, School of Medicine, Faculty of Health Sciences, The University of Adelaide, Royal Adelaide Hospital, South Australia 5005, Australia
通讯作者：
通讯机构：[1]Discipline of Surgery,School of Medicine,Faculty of Health Sciences,The University of Adelaide,Royal Adelaide Hospital,South Australia 5005,Australia[*1]Discipline of Surgery, School of Medicine, Faculty of Health Sciences, The University of Adelaide, Royal Adelaide Hospital, South Australia 5005, Australia
推荐引用方式(GB/T 7714)：
Smith Eric,De Young Neville J.,Tian Zi-Qiang,et al.Methylation of TIMP3 in esophageal squamous cell carcinoma[J].WORLD JOURNAL OF GASTROENTEROLOGY.2008,14(2):203-210.doi:10.3748/wjg.14.203.
APA：
Smith, Eric,De Young, Neville J.,Tian, Zi-Qiang,Caruso, Maria,Ruszkiewicz, Andrew R....&Drew, Paul A..(2008).Methylation of TIMP3 in esophageal squamous cell carcinoma.WORLD JOURNAL OF GASTROENTEROLOGY,14,(2)
MLA：
Smith, Eric,et al."Methylation of TIMP3 in esophageal squamous cell carcinoma".WORLD JOURNAL OF GASTROENTEROLOGY 14..2(2008):203-210
