机构：[1]Department of Gynecology, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China.临床科室妇科河北医科大学第四医院[2]Department of Gynecology and Obstetrics, Hebei General Hospital, Shijiazhuang 050000, China.[3]Department of Gynecology and General Medical Ward, Fourth Hospital of Hebei MedicalUniversity, Shijiazhuang 050011, China.[4]Department of Gynecology and Obstetrics, Fourth Hospital of Shijiazhuang, Shijiazhuang 050011, China.[5]Department of General Surgery, Hebei General Hospital, Shijiazhuang 050000, China. 6Research Center, Fourth Hospital of Hebei Medical University, No.12, Jiankang Road, Shijiazhuang 050011, China[6]Research Center, Fourth Hospital of Hebei Medical University, No.12, Jiankang Road, Shijiazhuang 050011, China.科研中心医技科室
ObjectiveTo identify the plasma protein biomarkers related to the chemoresistance of postoperative recurrence of epithelial ovarian cancers.MethodsForty plasma samples from patients in chemotherapy-sensitive and chemotherapy-resistant groups (20 for each group) were collected at Gynecology Department in the Fourth Hospital of Hebei Medical University from September 2013 to September 2014. The differentially expressed proteins between two groups were screened with two-dimensional gel electrophoresis (2-DE) and further analyzed by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS).ResultsThirty-four differentially expressed spots were identified between the two groups. Compared with the chemo-sensitive group, 21 protein spots were up-regulated and 13 were down-regulated in the chemoresistant group, in which 14 differentially expressed proteins were identified by the Mass spectrometry and Mascot search. Among the 14 proteins, complement C4-A, IgJ chain, clusterin, -1-antitrypsin and carbonic anhydrase 1 were up-regulated, and transthyretin, haptoglobin, -2-glycoprotein, Ig -2 chain C region, Ig -1 chain C region, complement factor I light chain, Ig chain C region, complement C3 and apolipoprotein E were down-regulated in the chemoresistant group when compared with the chemosensitive group.ConclusionThe up-regulated proteins including transthyretin, apolipoprotein E and haptoglobin proteins and the down-regulated proteins such as clusterin, carbonic anhydrase 1, alpha-1-antitrypsin were differentially expressed in the plasma between the chemo-sensitive group and the chemoresistant group, which may be potential biomarkers for predicting the chemotresistance of epithelial ovarian cancer patients.
基金：
Hebei Natural Science FoundationNatural Science Foundation of Hebei Province [H2018206347]; International Cooperation Project from Department of Science and Technology of Hebei Province [14397703D]; Capital Marathon Foundation of Chinese Cancer Association [LC2016W11]
第一作者机构：[1]Department of Gynecology, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China.
通讯作者：
通讯机构：[6]Research Center, Fourth Hospital of Hebei Medical University, No.12, Jiankang Road, Shijiazhuang 050011, China.
推荐引用方式(GB/T 7714)：
Zhang Zhengmao,Qin Kaiyun,Zhang Wenzhe,et al.Postoperative recurrence of epithelial ovarian cancer patients and chemoresistance related protein analyses[J].JOURNAL OF OVARIAN RESEARCH.2019,12:doi:10.1186/s13048-019-0499-z.
APA：
Zhang, Zhengmao,Qin, Kaiyun,Zhang, Wenzhe,Yang, Botao,Zhao, Chen...&Shan, Baoen.(2019).Postoperative recurrence of epithelial ovarian cancer patients and chemoresistance related protein analyses.JOURNAL OF OVARIAN RESEARCH,12,
MLA：
Zhang, Zhengmao,et al."Postoperative recurrence of epithelial ovarian cancer patients and chemoresistance related protein analyses".JOURNAL OF OVARIAN RESEARCH 12.(2019)
